Enzyme-assisted polymer film degradation-enabled biomolecule sensing with poly (N-isopropylacrylamide)-based optical devices

• Author(s) / Creator(s)

  • Zhang, Wei
  • Wei, Menglian
  • Carvalho, Wildemar S. P.
  • Serpe, Michael J.

• A biosensor for mouse Immunoglobulin G (IgG) was generated from responsive polymer-based interference filters (etalons). To accomplish this, an excess amount of alkaline phosphatase-modified goat anti-mouse IgG (AP-GAM, F(ab’)2 fragment specific to mouse IgG) was added to mouse IgG, and allowed to react for some time. After a given reaction time, the bound AP-GAM could be isolated from the unbound, excess AP-GAM by addition of goat anti-mouse IgG (Fc fragment specific)-modified magnetic microspheres (GAM-M) that bind the mouse IgG bound to AP-GAM. After application of a magnetic field, the free, unbound AP-GAM was isolated from the mixture and exposed to an etalon that has its upper Au surface modified with phosphate-containing polymer that can be degraded by AP-GAM. By the phosphate-containing polymer being degraded by the excess AP-GAM, the cleaved phosphate groups can diffuse into the interference filter's active polymer layer that yields a change in the optical properties that can be related to the amount of IgG in the sample. This concept is extremely straightforward to implement, and can be modified to detect a variety of other analytes of interest.

• Date created

  2018-01-01

• Subjects / Keywords

  • Sensing
  • Poly (N-Isopropylacrylamide)
  • Biosensing
  • Enzymatic Degradation
  • Etalons

• Type of Item

  Article (Draft / Submitted)

• DOI

  https://doi.org/10.7939/r3-pd9s-f424

• License

  © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. This is an open access article which is free to download and share.