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1989-01-01

Loppnow, Glen R., Mathies, Richard A.

A 77‐K cold stage has been developed for spectroscopic measurements in an optical microscope. This stage eliminates fogging of the optical windows observed in an earlier design by adding vacuum jackets around the liquid nitrogen transfer lines and by maximizing the sample‐to‐environment distance...

1998-01-01

Mitambo, Mitambo M., Zhang, Shuliang L., Loppnow, Glen R.

A novel four nuclear magnetic resonance (NMR)-tube holder is described for quantitative Raman and resonance Raman spectroscopy. This cell has advantages over other divided cell designs in producing high precision quantitation and eliminating off-axis errors. Raman spectra were obtained for both...

2002-01-01

Shoute, Lian C. T., Loppnow, Glen R.

Resonance Raman spectra of alizarin-sensitized TiO2 nanoparticles have been obtained at excitation wavelengths throughout the 488-nm charge transfer absorption band. The resonance Raman spectrum of the alizarin-sensitized TiO2 nanoparticle is significantly different from the spectrum of free...

2004

Okon, Mark, Schulman, Brenda A., Macauley, Matthew S., McIntosh, Lawrence P., Mackereth, Cameron D., Schärpf, Manuela, Errington, Wesley J.

Although sumoylation regulates a diverse and growing number of recognized biological processes, the molecular mechanisms by which the covalent attachment of the ubiquitin-like protein SUMO can alter the properties of a target protein remain to be established. To address this question, we have...

2005

Vocadlo, David J., Whitworth, Garrett E., Macauley, Matthew S., Debowski, Aleksandra W., Chin, Danielle

The post-translational modification of serine and threonine residues of nucleocytoplasmic proteins with 2-acetamido-2-deoxy-d-glucopyranose (GlcNAc) is a reversible process implicated in multiple cellular processes. The enzyme O-GlcNAcase catalyzes the cleavage of β-O-linked GlcNAc (O-GlcNAc)...

2006

Mackereth, Cameron D., Graves, Barbara J., Macauley, Matthew S., Schärpf, Manuela, McIntosh, Lawrence P. , Blaszczak, Adam G., Errington, Wesley J.

Sumoylation regulates the activities of several members of the ETS transcription factor family. To provide a molecular framework for understanding this regulation, we have characterized the conjugation of Ets-1 with SUMO-1. Ets-1 is modified in vivo predominantly at a consensus sumoylation motif...

2007

Fusco P. C., Bundle, D. R., Ling, C.-C., Michon, F., Moore, S. L., Uitz, C.

Previous studies have identified the length dependency of several polysaccharide (PS) protective epitopes. We have investigated whether meningococcal polysaccharides Y and W-135 possess such epitopes. Oligosaccharides (OSs) consisting of one or more disaccharide repeating units (RU) were derived...

2008

Bubb, Abigail K., Macauley, Matthew S., Davies, Gideon J., Vocadlo, David J. , Martinez-Fleites, Carlos

The O-GlcNAc post-translational modification is considered to act as a sensor of nutrient flux through the hexosamine biosynthetic pathway. A cornerstone of this hypothesis is that global elevation of protein O-GlcNAc levels, typically induced with the non-selective O-GlcNAcase inhibitor PUGNAc...

2009

Shoemaker, Glen K., Klassen, John S., Palcic, Monica M., Soya, Naoto

The first comparative thermodynamic study of the human blood group glycosyltransferases, α-(1→3)-N-acetylgalactosaminyltransferase (GTA) and α-(1→3)-galactosyltransferase (GTB), interacting with donor substrates, donor and acceptor analogs, and trisaccharide products in vitro is reported. The...

2009

Kitova, Elena N., Sun, Jiangxiao, Sun, Nian, Klassen, John S.

The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...