Search
Skip to Search Results- 14Agricultural, Food and Nutritional Science, Department of
- 14Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 10Graduate and Postdoctoral Studies (GPS), Faculty of
- 10Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 1InSciTE & Science 100
- 1InSciTE & Science 100/Capstone Project Posters (Science 100)
- 2Deyholos, Michael (Biological Sciences)
- 1Chen, Guanqun (Department of Agricultural, Food and Nutritional Science)
- 1Davis, Corey (Biological Sciences)
- 1Oba, Masahito (Agricultural, Food, and Nutritional Science)
- 1Ozga, Jocelyn (Department of Agricultural, Food and Nutritional Science)
- 1Sauvageau, Dominic (Chemical and Materials Engineering)
-
In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
-
Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein
Download2020-01-01
Xu, Yang, Caldo, Kristian Mark P., Falarz, Lucas, Jayawardhane, Kethmi, Chen, Guanqun
SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes the final step of acyl-CoA-dependent biosynthesis of TAG and is considered a key target for manipulating oil production....
-
Mammalian and Saccharomyces cerevisiae ER-mitochondria contact site regulation by small Rab GTPases and ER folding assistants
DownloadFall 2021
Membrane-bound organelles allow eukaryotes to compartmentalize components and processes in a highly organized manner. Organelles can communicate with one another through membrane contact sites (MCS): membrane appositions 10-50nm apart. MCS were not widely accepted as bona fide sites of organelle...
-
Mechanisms of inactivation of Candida humilis and Saccharomyces cerevisiae by pulsed electric fields
Download2017-06-01
Ou, Qi-Xing, Nikolic-Jaric, Marija, Gänzle, Michael
Aims This study aimed to determine how electric field strength, pulse width and shape, and specific energy input relate to the effect of pulsed electric fields (PEF) on viability and membrane permeabilization in Candida humilis and Saccharomyces cerevisiae suspended in potassium phosphate...
-
Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...
-
Fall 2024
Punicic acid (PuA) is a high-value edible conjugated fatty acid with strong bioactivities and has important potential applications in nutraceutical, pharmaceutical, and oleochemical industries. Since the production of PuA is severely limited by the fact that its primary natural source,...
-
Properties and Biotechnological Applications of Acyl‐CoA:diacylglycerol Acyltransferase and Phospholipid:diacylglycerol Acyltransferase from Terrestrial Plants and Microalgae
Download2018-07-30
Xu, Yang, Caldo, Kristian Mark P., Pal‐Nath, Dipasmita, Ozga, Jocelyn, Lemieux, M. Joanne, Weselake, Randall J., Chen, Guanqun
Triacylglycerol (TAG) is the major storage lipid in most terrestrial plants and microalgae, and has great nutritional and industrial value. Since the demand for vegetable oil is consistently increasing, numerous studies have been focused on improving the TAG content and modifying the fatty‐acid...
-
Spring 2022
Self-cycling fermentation (SCF) is an advanced, automated, semi-continuous fermentation strategy that is used to improve the volumetric productivity of bioproduction. Typically, a microbial culture is grown in a reactor and half the reactor contents are harvested once a limiting nutrient is...
-
STRESS RESPONSES OF THE FLAX GENOME: ACTIVATION OF TRANSPOSABLE ELEMENTS, DEFENSE GENES AND GENOME RESTRUCTURING AND DIVERSIFICATION.
DownloadSpring 2017
Transposable elements (TEs) are DNA sequences that can move in the genome (transpose) via a DNA or a RNA intermediate. TEs are abundant in plant genomes and can be activated by stress. Their activity can result in structural and gene expression alterations that increase diversity within and among...
-
Substrate preferences of long-chain acyl-CoA synthetase and diacylglycerol acyltransferase contribute to enrichment of flax seed oil with a-linolenic acid
Download2018-04-01
Xu, Yang, Holic, Roman, Li, Darren, Pan, Xue, Mietkiewska, Elzbieta, Chen, Guanqun, Ozga, Jocelyn, Weselake, Randall J.
Seed oil from flax (Linum usitatissimum) is enriched in a-linolenic acid (ALA; 18:3?9cis,12cis,15cis), but the biochemical processes underlying the enrichment of flax seed oil with this polyunsaturated fatty acid are not fully elucidated. Here, a potential process involving the catalytic actions...