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Skip to Search Results- 14Agricultural, Food and Nutritional Science, Department of
- 14Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 14Graduate and Postdoctoral Studies (GPS), Faculty of
- 14Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 1InSciTE & Science 100
- 1InSciTE & Science 100/Capstone Project Posters (Science 100)
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Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and DGAT activity
Download2021-01-01
Xu, Yang, Pan, Xue, Lu, Junhao, Wang, Juli, Shan, Qiyuan, Stout, Jake, Chen, Guanqun
Abstract: Wax synthase (WS) catalyzes the last step in wax ester biosynthesis in green plants. Two unrelated subfamilies of WS including the bifunctional acyltransferase and plant-like WS have been reported, but the latter is largely uncharacterized in microalgae. Here, we functionally...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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Improving the production of punicic acid in baker’s yeast by engineering genes in acyl channeling processes and adjusting precursor supply
Download2021-01-01
Wang, Juli, Xu, Yang, Holic, Roman, Yu, Xiaochen, Singer, Stacy D., Chen, Guanqun
ABSTRACT Punicic acid (PuA) is a high-value edible conjugated fatty acid with strong bioactivities and has important potential applications in nutraceutical, pharmaceutical, feeding and oleochemical industries. Since the production of PuA is severely limited by the fact that its natural source...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein
Download2020-01-01
Xu, Yang, Caldo, Kristian Mark P., Falarz, Lucas, Jayawardhane, Kethmi, Chen, Guanqun
SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes the final step of acyl-CoA-dependent biosynthesis of TAG and is considered a key target for manipulating oil production....
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Mammalian and Saccharomyces cerevisiae ER-mitochondria contact site regulation by small Rab GTPases and ER folding assistants
DownloadFall 2021
Membrane-bound organelles allow eukaryotes to compartmentalize components and processes in a highly organized manner. Organelles can communicate with one another through membrane contact sites (MCS): membrane appositions 10-50nm apart. MCS were not widely accepted as bona fide sites of organelle...
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Mechanisms of inactivation of Candida humilis and Saccharomyces cerevisiae by pulsed electric fields
Download2017-06-01
Ou, Qi-Xing, Nikolic-Jaric, Marija, Gänzle, Michael
Aims This study aimed to determine how electric field strength, pulse width and shape, and specific energy input relate to the effect of pulsed electric fields (PEF) on viability and membrane permeabilization in Candida humilis and Saccharomyces cerevisiae suspended in potassium phosphate...
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Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...