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Skip to Search Results- 15Chen, Guanqun
- 9Singer, Stacy D.
- 7Xu, Yang
- 5Weselake, Randall J.
- 3Caldo, Kristian Mark P.
- 3Jayawardhane, Kethmi N.
- 3Arabidopsis thaliana
- 2CRISPR/Cas
- 2Ricinoleic acid
- 2Saccharomyces cerevisiae
- 2Triacylglycerol
- 2biotechnology
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A Fluorescence‐Based Assay for Quantitative Analysis of Phospholipid:Diacylglycerol Acyltransferase Activity
Download2019-09-02
Falarz, Lucas, Xu, Yang, Singer, Stacy D., Chen, Guanqun
Phospholipid:diacylglycerol acyltransferase (PDAT) catalyzes the acyl‐CoA‐independent triacylglycerol (TAG) biosynthesis in plants and oleaginous microorganisms and thus is a key target in lipid research. The conventional in vitro PDAT activity assay involves the use of radiolabeled substrates,...
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2020-03-01
Tian, Bo, Sun, Meijuan, Jayawardana, Kethmi, Wu, Ding, Chen, Guanqun
Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri‐ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n‐9) esterified to the sn‐2 position of phosphatidylcholine (PtdCho) catalyzed by...
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Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme
Download2019-12-26
Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
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Characterization of the diversification of phospholipid:diacylglycerol acyltransferases in the green lineage
Download2020-06-15
Falarz, Lucas J., Xu, Yang, Caldo, Kristian Mark P., Garroway, Colin J., Singer, Stacy D., Chen, Guanqun
Triacylglycerols have important physiological roles in photosynthetic organisms, and are widely used as food, feed and industrial materials in our daily life. Phospholipid:diacylglycerol acyltransferase (PDAT) is the pivotal enzyme catalyzing the acyl‐CoA‐independent biosynthesis of...
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2020-06-13
Subedi, Udaya, Ozga, Jocelyn A., Chen, Guanqun, Foroud, Nora A., Singer, Stacy D.
The demand for vegetable oils is increasing at a rapid pace due to our ever-expanding population, growing global affluence, changing dietary choices, and the need for renewable plant-derived resources. However, oilseed production is negatively impacted by unpredictable environmental conditions...
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Downregulation of key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissue
Download2020-02-11
Wijekoon, Champa, Singer, Stacy D., Weselake, Randall J., Petrie, James R., Chen, Guanqun, Singh, Surinder, Eastmond, Peter J., Acharya, Surya N.
Alfalfa (Medicago sativa L.) is the most widely grown perennial forage crop and is a close relative of the model diploid legume Medicago truncatula Gaertn. However, use of alfalfa leads to substantial greenhouse gas emissions and economic losses related to inefficiencies in rumen fermentation....
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Engineering Arabidopsis long-chain acyl-CoA synthetase 9 variants with enhanced enzyme activity
Download2018-12-17
Xu, Yang, Caldo, Kristian Mark P., Holic, Roman, Mietkiewska, Elzbieta, Ozga, Jocelyn, Rizvi, Syed Masood, Chen, Guanqun, Weselake, Randall
Long-chain acyl-CoA synthetase (LACS, EC 6.2.1.3) catalyzes the ATP-dependent activation of free fatty acid to form acyl-CoA, which in turn serves as the major acyl donor for various lipid metabolic pathways. Increasing the size of acyl-CoA pool by enhancing LACS activity appears to be a useful...
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Enhancement of total lipid production in vegetative tissues of alfalfa and sainfoin using chemical mutagenesis
Download2019-12-31
Wijekoon, Champa P., Singer, Stacy D., Weselake, Randall J., Petrie, James R., Singh, Surinder, Jayawardhane, Kethmi N., Shah, Saleh, Chen, Guanqun, Eastmond, Peter J., Acharya, Surya N.
Alfalfa (Medicago sativa L.) and sainfoin (Onobrychis viciifoila Scop.) are two key forage legumes for the western Canadian cattle industry. Despite the high protein content, drawbacks to their use exists, including inefficient protein digestibility and energy use efficiency in the ruminant...
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Feeding a Bioactive Oil Enriched in Stearidonic Acid during Early Life Influences Immune System Maturation in Neonatal Sprague-Dawley Rats
Download2020-03-01
Patel, Dhruvesh, Goruk, Susan, Newell, Marnie, Chen, Guanqun, Richard, Caroline, Field, Catherine J.
Long-chain n-3 PUFAs (LCPUFAs) improve immune development and reduce atopic disease risk in infants. Stearidonic acid (SDA) can be a substrate for biosynthesis of n-3 LCPUFAs.<bold>Objective: </bold>We aimed to determine the effect of feeding an SDA-enriched diet during suckling and weaning on...
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Identification of genes associated with ricinoleic acid accumulation in Hiptage benghalensis via transcriptome analysis
Download2018-12-16
Tian, Bo, Lu, Tianquan, Xu, Yang, Wang, Ruling, Chen, Guanqun
Background: Ricinoleic acid is a high-value hydroxy fatty acid with broad industrial applications.Hiptage benghalensis seed oil contains a high amount of ricinoleic acid (~80%) and represents an emerging source of this unusual fatty acid. However, the mechanism of ricinoleic acid accumulation in...