J-Coupling Effects on Quantification of the 1.3 ppm Lipid Methylene Resonance with In-Vivo Magnetic Resonance Spectroscopy at 3 T

• Author / Creator

  Breitkreutz, Dylan Y

• Quantification of 1.3 ppm lipid methylene protons by proton magnetic resonance spectroscopy has been shown to be relevant to a number of diseases. Additionally, T2 estimation of 1.3 ppm methylene protons has been used to grade the metastatic potential of colon tumours. J-coupling interactions of lipid protons result in time dependent signal modulations. J-coupling effects can hinder quantification and T2 determination. The objective of the work presented in this thesis is to investigate the effects J-coupling on quantification and T2 estimation of 1.3 ppm lipid methylene protons by using a modified PRESS sequence to rewind the J-coupling evolution. The effects of J-coupling on quantification and T2 determination are studied by comparing the response of fatty acid phantoms and the tibial bone marrow of six volunteers to a standard PRESS sequence and to a modified PRESS sequence, which is designed to rewind J-coupling evolution. The work demonstrates that the consequence of neglecting to consider J-coupling effects on the quantification of 1.3 ppm lipid methylene protons and on their T2 values is not negligible.

• Subjects / Keywords

  • MRS
  • NMR
  • Quantification
  • J-coupling
  • Lipids
  • Methylene

• Graduation date

  Fall 2015

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3J38KV61

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.