Vitamin D, an Immunomodulator for Eosinophils in Asthma and Allergy

  • Author / Creator
    Ethier, Caroline D
  • Epidemiological studies correlate vitamin D deficiency with asthma severity. Calcitriol modulates receptor and cytokine expression from various leukocytes in an anti-inflammatory manner. Despite eosinophil activity during mucosal inflammation, knowledge on the direct effect of calcitriol on this granulocyte is very limited. We propose that calcitriol exerts direct effects on eosinophil biology by modulating its programmed cell death pathways and other functions. Purified peripheral blood eosinophils from atopic donors were incubated with calcitriol (10nM) for up to 14 days with and without IL-5. We observed that calcitriol significantly reduced eosinophil necrosis and cytolytic release of EPX in media when co-incubated with IL-5. The addition of calcitriol also significantly decreased CRTh2 and CCR10 expression on eosinophils. Eosinophil cytotoxic mediator release into mucosal tissues, resulting from necrotic cytolysis, as well as eosinophil recruitment to lungs are major contributors to airway inflammation in allergic asthma. Hence, the reduction of mucosal inflammation achieved by decreasing eosinophil necrosis and recruitment unveil new anti-inflammatory mechanisms for calcitriol in the context of airway inflammation.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Medicine
  • Specialization
    • Experimental Medicine
  • Supervisor / co-supervisor and their department(s)
    • Cameron, Lisa (Department of Medicine)
    • Davoine, Francis (Department of Medicine and Faculte Saint-Jean)
  • Examining committee members and their departments
    • Mazurak, Vera (Department of Agricultural, Food & Nutritional Science)
    • Davoine, Francis (Department of Medicine and Faculte Saint-Jean)
    • Cameron, Lisa (Department of Medicine)
    • Lacy, Paige (Department of Medicine)