Enhancing chondrogenesis of mesenchymal stromal cells through anti-Thy1 strategies

• Author / Creator

  Yu, Hana

• The intricate arrangement and composition of articular cartilage confer the structure its unique material and mechanical properties. Degeneration of articular cartilage, especially osteoarthritis, imposes great burden not only on the patients, but also on society. While there exist various non-surgical and surgical treatment methods, continuous research is required to overcome the limitations of current therapeutic methods.

  Mesenchymal stromal cell (MSC)-based therapy of cartilage defects has been developed to generate replacement articular chondrocytes for the production of extra cellular matrix. To develop a novel way of advancing MSC-based therapy, we examined the chondrogenic potential of MSCs that have undergone anti-Thy1 treatments. Although several studies have suggested a potential link between Thy-1 and chondrogenic potential of MSCs, our anti-Thy1 strategy did not enhance chondrogenic potential of MSCs. Furthermore, we report that a common lentiviral transduction strategy can compromise MSCs’ chondrogenic capacity, and that Thy-1 expression during chondrogenesis is dynamic in its nature.

• Subjects / Keywords

  • Mesenchymal stem cells
  • Thy-1
  • Chondrogenesis
  • CD90

• Graduation date

  Spring 2014

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R35Q45

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.