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Broadening of genetic diversity in spring canola (Brassica napus L.) by use of yellow sarson and Canadian spring Brassica rapa L.
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- Author / Creator
- Attri, Rohit
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Canada is the top producer of Brassica oilseeds [B. napus L. (n = 19, AC genome)] in the world. Genetic diversity has declined in this crop in the recent years due to use of only superior and genetically narrow gene pool in breeding. Presence of adequate genetic diversity is important for further improvement of this crop through breeding. Genetically distinct germplasm of B. napus or its allied species can be used to broaden genetic diversity in Canadian B. napus canola. However, limited efforts have been made to utilize genetic diversity of the progenitor species B. rapa (n = 10, A genome) and B. oleracea (n = 9, C genome) in the breeding of this crop as interspecific cross often introduces undesirable traits in the breeding program. This M.Sc. thesis research was undertaken to develop genetically distinct B. napus lines through interspecific crosses between B. napus canola and B. rapa. For this, three genetically distinct B. rapa lines were used. The F1’s of B. napus × B. rapa interspecific crosses were self-pollinated for F2 as well as backcrossed to the B. napus parent for BC1F1 progenies. Pedigree breeding was applied where selection for plant fertility and glucosinolate content was done in each generation. SSR marker analysis of the F4 plants revealed that the three populations derived from B. napus × B. rapa crosses are genetically distinct from each other as well as from the B. napus parent; thus, the advanced generation populations derived from the progeny of these plants expected to carry allelic diversity of the B. rapa parents. Plant fertility and glucosinolates content in many of the F7 and BC1F4 families reached close to the B. napus parent. Flow cytometric analysis of F6 and BC1F3 families for nuclear DNA content indicated that many families are euploid B. napus type. Findings from this thesis research suggest that genetically distinct, fertile, euploid B. napus canola lines can be developed from both F2 and BC1F1 of the B. napus × B. rapa interspecific crosses.
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- Graduation date
- Fall 2015
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.