The vaccinia virus N2 protein associates with karyopherins α2 and α4 and reduces the turnover rate of karyopherin α2

  • Author / Creator
    Desaulniers, Megan Amanda
  • Due to their large genomes, poxviruses encode a number of enzymes, including a DNA polymerase and a DNA-dependent RNA polymerase, and therefore require few host gene factors for their replication. Several studies have shown several host nuclear factors are in fact recruited to viral sites of replication. Using bioinformatics we identified a putative bipartite nuclear localization signal in vaccinia virus N2L. Through immuno-precipitation, we were able to show that N2 interacts with two nuclear import proteins, karyopherins (KPN) alpha 2 and alpha 4. Immunofluorescence analysis indicated that in the presence of N2, KPNα2 was found evenly dispersed throughout the cell. However, when N2 is absent from the infection, KPNα2 accumulates at the nuclear periphery. Using Fluorescence Recovery After Photobleaching (FRAP), we show that the presence of N2 retards the nuclear turnover rate of KPNα2. Taken together, we suggest that N2 is competing for available KPNα2 to modulate nuclear transport thus promoting virulence.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Medical Microbiology and Immunology
  • Specialization
    • Virology
  • Supervisor / co-supervisor and their department(s)
    • Evans, David (Medical Microbiology and Immunology)
  • Examining committee members and their departments
    • Schang, Luis (Biochemistry)
    • Smiley, Jim (Medical Microbiology and Immunology)
    • Shmulevitz, Maya (Medical Microbiology and Immunology)