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- 1Desaulniers, Megan Amanda
- 1Gammon, Donald Brad
- 1Harrison, Melissa L
- 1Irwin, Chad Robert
- 1Kieser, Quinten J
- 1Paszkowski, Patrick
Investigating barriers to vaccinia virus recombination using combinations of light and electron microscopyDownload
While DNA viruses typically replicate in the nucleus of the host cell, poxvirus replication occurs exclusively within cytoplasmic structures known as viral factories. Viral factories are the sites of various processes of the vaccinia virus (VACV) life cycle including replication, morphogenesis...
Deletion of F4L (ribonucleotide reductase) in Vaccinia Virus Produces a Selective Oncolytic Virus and Promotes Anti-tumor Immunity with Superior Safety in Bladder CancerDownload
Overall recurrence of non-muscle-invasive bladder cancer (NMIBC) can be as high as 80% within 5 years of initial treatment. High-grade NMIBC has the greatest risk of recurrence and treatment for these patients includes surgery followed by intravesical therapy with the immunotherapeutic agent...
Determination of Oligomeric State and Role of the Acidic C Terminal Tail of Vaccinia Virus I3 Single Stranded DNA Binding ProteinDownload
Single-stranded DNA (ssDNA) binding proteins (SSB) play a major role in DNA replication, recombination, and repair, by protecting ssDNA from nuclease attack and removing inhibitory secondary structure. Poxviruses are large double stranded DNA viruses that replicate within the cytoplasm of cells,...
Vaccinia virus (VACV) has shown promise as an oncolytic agent for treating a variety of tumour types, with preliminary results suggesting that this strategy holds promise for treating breast cancer. Our lab has previously modified VACV by deleting virally-encoded enzymes responsible for dNTP...
Investigations into Differences between Vaccinia and Myxoma Virus Plaquing Properties Identifies Strategies for Increasing the Oncolytic Efficacy of Myxoma VirusDownload
Despite both being poxviruses, vaccinia (VACV) and myxoma (MYXV) form very different plaque types. VACV plaques are large and show a central clearing of cells, while MYXV plaques are smaller and result in a clumping of cells. VACV spread is promoted by the formation of an enveloped form of virus...
The vaccinia virus N2 protein associates with karyopherins α2 and α4 and reduces the turnover rate of karyopherin α2Download
Due to their large genomes, poxviruses encode a number of enzymes, including a DNA polymerase and a DNA-dependent RNA polymerase, and therefore require few host gene factors for their replication. Several studies have shown several host nuclear factors are in fact recruited to viral sites of...
Recombination between co-infecting poxviruses provides an important mechanism for generating genetic diversity in the face of selection pressures. However, poxviruses replicate in membrane-bound enclosed cytoplasmic structures known as factories or virosomes that could impede DNA mixing between...
Poxviruses encode many genes that are orthologs of cellular genes. These orthologs serve many functions, but those that are of most interest are ones that have evolved further and now serve an immune-evasion function. Such genes were likely first acquired by poxviruses through some form of...
Vaccinia virus DNA polymerase and ribonucleotide reductase: their role in replication, recombination and drug resistanceDownload
Despite the eradication of smallpox, poxviruses continue to cause human disease around the world. At the core of poxvirus replication is the efficient and accurate synthesis and repair of the viral genome. The viral DNA polymerase is critical for these processes. Acyclic nucleoside phosphonate...