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Skip to Search Results- 4Michaelis, V.K.
- 2Bernard, G.M.
- 2Hwang, Peter M.
- 2Pan, Jonathan S.
- 1Ha, M and Michaelis, V.K.
- 1Ha, M.
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A PagP fusion protein system for the expression of intrinsically disordered proteins in E. coli
Download2012-01-01
Hwang, Peter M., Pan, Jonathan S., Sykes, Brian D.
PagP, a beta-barrel membrane protein found in Gram-negative bacteria, expresses robustly in inclusion bodies when its signal sequence is removed. We have developed a new fusion protein expression system based on PagP and demonstrated its utility in the expression of the unstructured N-terminal...
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Combining a PagP fusion protein system with nickel ion-catalyzed cleavage to produce intrinsically disordered proteins in E. coli
Download2015-01-01
Zahran, Somaya, Pan, Jonathan S., Liu, Philip B., Hwang, Peter M.
Many proteins contain intrinsically disordered regions that are highly solvent-exposed and susceptible to post-translational modifications. Studying these protein segments is critical to understanding their physiologic regulation, but proteolytic degradation can make them difficult to express and...
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2020-01-01
Hooper, R.W., Klein, B.A., Michaelis, V.K.
The design of new materials requires a deep understanding of the intricacies underpinning their structure–property relationships. These relationships are modified through synthesis and processing to create materials with new properties and characteristics to achieve a wide range of applications....
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2019-01-01
Ha, M., Thiessen, A.N., Sergeyev, I.V., Veinot, J.G.C., Michaelis, V.K.
Silicon nanoparticles (SiNPs) are intriguing materials and their properties fascinate the broader scientific community; they are also attractive to the biological and materials science sub-disciplines because of their established biological and environmental compatibility, as well as their...
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2017-05-04
Dynamic nuclear polarization (DNP) NMR spectroscopy, a high-polarization method, is rapidly changing the landscape of what is possible in solid-state nuclear magnetic resonance spectroscopy. To date, there have been over 200 publications discussing high-frequency DNP NMR of solids with more than...
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High-Frequency Dynamic Nuclear Polarization NMR for Solids: Part 2 – Development and Applications
Download2017-01-01
High-frequency dynamic nuclear polarization nuclear magnetic resonance (DNP NMR) spectroscopy is having a major impact on the far-reaching abilities of solid-state NMR. This high-sensitivity technique made possible by transferring high polarization from an unpaired electron source to an...
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2019-01-01
Bernard, G.M., Michaelis, V.K.
High‐field dynamic nuclear polarization nuclear magnetic resonance (DNP NMR) spectroscopy is rapidly emerging as a revolutionary analytical structural method by greatly enhancing the sensitivity of nuclear magnetic resonance. Innovative solutions are constantly being introduced to address the...
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Lead‐207 NMR spectroscopy at 1.4 T: Application of benchtop instrumentation to a challenging I = ½ nucleus
Download2020-01-01
Bernard, G.M., Michaelis, V.K.
The practicality of obtaining liquid‐ and solid‐state 207Pb nuclear magnetic resonance (NMR) spectra with a low permanent‐field magnet is investigated. Obtaining 207Pb NMR spectra of salts in solution is shown to be viable for samples as dilute as 0.05 M. The concentration dependence of the 207Pb...