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- 10Triacylglycerol
- 4DGAT
- 4Yeast
- 3Linum usitatissimum
- 2Arabidopsis-thaliana
- 6Weselake, Randall J.
- 4Chen, Guanqun
- 3Caldo, Kristian Mark P.
- 3Greer, Michael S.
- 3Mietkiewska, Elzbieta
- 3Pan, Xue
- 9Graduate and Postdoctoral Studies (GPS), Faculty of
- 9Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 8Agricultural, Food and Nutritional Science, Department of
- 8Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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- 7Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 3Department of Agricultural, Food, and Nutritional Science
- 1Department of Biochemistry
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- 1Douglas, Donna (Department of Surgery)
- 1Gänzle, Michael (Agricultural, Food and Nutritional Science)
- 1Kneteman, Norman (Department of Surgery)
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- 1Weselake, Randall (Department of Agricultural, Food and Nutritional Science)
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A 10 kDa acyl-CoA binding protein (ACBP) from Brassica napus enhances acyl exchange between acyl-CoA and phosphatidylcholine
Download2009
Yurchenko, O. P., Stymne, S., Stahl, U., Moloney, M. M., Nykiforuk, C. L., Weselake, R. J. , Banas, A.
The gene encoding a 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus wasover-expressed in developing seeds of Arabidopsis thaliana. Biochemical analysis of T2andT3A. thaliana seeds revealed a significant increase in polyunsaturated fatty acids (FAs)(18:2cisD9,12and 18:3cisD9,12,15) at...
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Biogenesis of Porin of the Outer Mitochondrial Membrane Involves an Import Pathway via Receptors and the General Import Pore of the TOM Complex
Download2001
Nargang, F. E., Pfanner, N., Neupert, W., Blachly-Dyson, E., Forte, M., Krimmer, T., Ryan, M. T., Kassenbrock, K., Rapaport, D., Douglas, M. G., Meisinger, C.
Abstract: Porin, also termed the voltage-dependent anion channel, is the most abundant protein of the mitochondrial outer membrane. The process of import and assembly of the protein is known to be dependent on the surface receptor Tom20, but the requirement for other mitochondrial proteins...
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2020-03-01
Tian, Bo, Sun, Meijuan, Jayawardana, Kethmi, Wu, Ding, Chen, Guanqun
Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri‐ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n‐9) esterified to the sn‐2 position of phosphatidylcholine (PtdCho) catalyzed by...
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Spring 2014
Metabolomics involves the high throughput characterization of small molecules or metabolites in cells, tissues and organisms. To interpret, store and exchange metabolomic data it is necessary to have comprehensive, electronically accessible databases that can be used to handle both the...
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis
Download2011
Siloto, Rodrigo M. P., Snyder, Crystal L., Liu, Qin, Weselake, Randall J.
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...
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Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
Download2007
Pozniakovsky, A., Hyman, A.A., Stirling, P.C., Takhar, K.S., Srayko, M., Lerou, M.R.
The Chaperonin Containing Tcp1 (CCT) maintains cellular protein folding homeostasis in the eukaryotic cytosol by assisting the biogenesis of many proteins, including actins, tubulins, and regulators of the cell cycle. Here, we demonstrate that the essential and conserved eukaryotic phosducin-like...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...