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Skip to Search Results- 5Weselake, Randall J.
- 2Neupert, W.
- 2Siloto, Rodrigo M. P.
- 2Snyder, Crystal L.
- 2Xu, Yang
- 1 Lemieux, M. Joanne
- 12Graduate and Postdoctoral Studies (GPS), Faculty of
- 12Graduate and Postdoctoral Studies (GPS), Faculty of /Theses and Dissertations
- 9Biological Sciences, Department of
- 9Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 6Agricultural, Food and Nutritional Science, Department of
- 6Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 4Department of Agricultural, Food, and Nutritional Science
- 2Department of Biochemistry
- 2Department of Biological Sciences
- 1Department of Chemical and Materials Engineering
- 1Department of Chemistry
- 1Department of Computing Science
- 1Gänzle, Michael (Agricultural, Food and Nutritional Science)
- 1James, Michael N. G. (Biochemistry)
- 1Li, Liang (Chemistry)
- 1Ozga, Jocelyn (Department of Agricultural, Food and Nutritional Science)
- 1Sauvageau, Dominic (Chemical and Materials Engineering)
- 1Scarpella, Enrico (Biological Sciences)
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...
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Inactivation of Saccharomyces cerevisiae and Candida humilis in Potassium Phosphate Buffer by Pulsed Electric Fields
DownloadSpring 2016
Pulsed electric field (PEF) is a promising athermal food preservation technology, having the potential to provide a better balance between food safety and food quality. But the high cost of PEF treatment has limited the commercial application of this technology. The aim of this MSc thesis...
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Functional Characterization of Plant Patatin-like Phospholipase A III in Transgenic Arabidopsis thaliana Producing 18 Carbon Hydroxy Fatty Acids
DownloadFall 2016
Hydroxy fatty acid (HFA)-enriched oils are valuable in many oleo-chemical industries. The major natural source of HFA, however, is castor (Ricinus communis) seed, which is poisonous to human beings and animals. Although over-expressing a castor OLEATE 12-HYDROXYLASE (RcFAH12) in Arabiodpsis...
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Development and Application of Isotope Labeling Method and Data Processing Program for Liquid Chromatography Mass Spectrometry Based Metabolome Profiling
DownloadSpring 2015
The objective of this thesis focuses on establishing a software tool (IsoMS) used in the quantification and qualification of metabolites in complex biological samples, developing robust methods to extract metabolites from different plant samples, and applying a differential 13C-/12C-isotope...
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Type-1 Brassica napus Diacylglycerol Acyltransferases: Enzyme Characterization and Molecular Tools for Increasing Storage Lipid Production in Yeast
DownloadFall 2015
Diacylglycerol acyltransferase (DGAT) catalyzes the final step in the acyl-CoA- dependent biosynthesis of triacylglycerol (TAG). The level of DGAT activity may have a substantial effect on the flow of carbon into TAG in many organisms. In plants, yeast, and animals, two families of...
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The Impact of Self-Cycling Fermentation on the Production of Shikimic Acid in Populations of Engineered Saccharomyces cerevisiae
DownloadFall 2015
Shikimic acid is an intermediary metabolite in the biosynthesis of aromatic amino acids in plants and microorganisms. It is also a precursor for the production of specialty chemical ingredients used in pharmaceutical, cosmetic, and healthcare products. However, the current commercial method of...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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Differential expression of miRNAs in Brassica napus root following infection with Plasmodiophora brassicae
Download2014
Basu, Urmila, Verma, Shiv, Rahman, Muhammad H., Deyholos, Michael K., Kav, Nat N. V.
Canola (oilseed rape, Brassica napus L.) is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have...
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Spring 2014
Metabolomics involves the high throughput characterization of small molecules or metabolites in cells, tissues and organisms. To interpret, store and exchange metabolomic data it is necessary to have comprehensive, electronically accessible databases that can be used to handle both the...