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2003

Day, Andy E., Andersson, Leif, Plastow, Graham S., Pielberg, Gerli

Gene duplications are common in the vertebrate genome, and duplicated loci often show a variation in copy number that may have important phenotypic effects. Here we describe a powerful method for quantification of duplicated copies based on pyrosequencing. A reliable quantification was obtained...

1995

Hickey, D. A., Sperling, F. A. H.

Abstract: We describe a method for identifying conifer-feeding species and lineages of Choristoneura Lederer in Canada and Alaska. The method relies on amplification of mitochondrial (mt) DNA by the polymerase chain reaction (PCR); amplified DNA is then digested with restriction enzymes to give...

2002

Fitzsimmons, C. J., Thue, T. D., Buchanan, F. C., Winkelman-Sim, D. C., Schmutz, S. M., Van Kessel, A. G.

Previously, we have shown that alleles of the BM1500 microsatellite, located 3.6 kb downstream of the leptin gene in cattle, were associated with carcass fat measures in a population of 154 unrelated beef bulls. Subsequently, a cytosine (C) to thymine (T) transition that encoded an amino acid...

2004

Srayko, M., Mains, P.E., Lu, C.

The microtubule-severing protein complex katanin is required for a variety of important microtubule-base morphological changes in both animals and plants. Caenorhabditis elegans katanin is encoded by the mei-1 and mei-2 genes and is required for oocyte meiotic spindle formation and must be...

2023-08-11

Essienton, Edidiong

Fall 2011

Karami, Jamil

This thesis deals with finding design points for nonlinear regression models with the possibility that the fitted model is incorrect. The information matrix depends on the parameter in nonlinear situations. We have assumed a range of values of the parameter and have specified a prior on the...

Spring 2015

Northrup, Victoria A.

Mutations in the mitochondrial genome (mtDNA) have been linked to a wide variety of disorders. mtDNA analyses require large starting samples and are complicated by the presence of nuclear mitochondrial pseudogenes (NUMTs), which can cause false positives in PCR-based approaches. Microfluidic...

1998

Day, B. W., Reha-Krantz, L., Shukla, R., Melacrinos, A., Keohavong, P.

Abstract: In vitro DNA replication by exonuclease-deficient T7 DNA polymerase (Sequenase) and an exonuclease deficient T4 DNA polymerase was examined on a 244-nucleotide DNA template treated with three electrophilic polycyclic aromatic hydrocarbon (PAH) metabolites: racemic...

Fall 2014

Duguay, Brett A K

Infection with herpes simplex virus type 1 (HSV-1) leads to the rapid and complete loss of mitochondrial DNA (mtDNA) and mitochondrial messenger RNA (mt-mRNA), effectively eliminating gene expression within mitochondria. Previous work determined that a unique 3’ co-terminal transcript arising...

2014

Bamforth, Fiona, Stothard, Paul, Peng, Fred, Sergi, Consolato, Chan, Alicia, Bahitham, Wesam, Stone, Bradley, Mason, Andrew, Liao, Xiaoping

Cholangiocellular carcinoma (CCA) of the liver was the target of more interest, recently, due mainly to its increased incidence and possible association to new environmental factors. Somatic mitochondrial DNA (mtDNA) mutations have been found in several cancers. Some of these malignancies contain...