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Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels

  • Author(s) / Creator(s)
  • Previously, we have shown that alleles of the BM1500 microsatellite, located 3.6 kb downstream of the leptin gene in cattle, were associated with carcass fat measures in a population of 154 unrelated beef bulls. Subsequently, a cytosine (C) to thymine (T) transition that encoded an amino acid change of an arginine to a cysteine was identified in exon 2 of the leptin gene. A PCR-RFLP was designed and allele frequencies in four beef breeds were correlated with levels of carcass fat. The T allele was associated with fatter carcasses and the C allele with leaner carcasses. The frequencies of the SNP alleles among breeds indicated that British breeds have a higher frequency of the T allele whereas the continental breeds have a higher occurrence of the C allele. A ribonuclease protection assay was developed to quantify leptin mRNA in a separate group of animals selected by genotype. Animals homozygous for thymine expressed higher levels of leptin mRNA. This may suggest that the T allele, which adds an extra cysteine to the protein, imparts a partial loss of biological function and hence could be the causative mutation.

  • Date created
    2002
  • Subjects / Keywords
  • Type of Item
    Article (Published)
  • DOI
    https://doi.org/10.7939/R37P8TG05
  • License
    Attribution 4.0 International
  • Language
  • Citation for previous publication
    • Buchanan, F.C., Fitzsimmons, C.J, Van Kessel, A.G., Thue TD, Winkelman-Sim DC, & Schmutz SM. (2002). Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. GSE: Genetics, Selection, Evolution, 34(1), 105-116. http://dx.doi.org/10.1186/1297-9686-34-1-105
  • Link to related item
    http://dx.doi.org/10.1186/1297-9686-34-1-105