Search
Skip to Search Results- 8Mass-spectrometry
- 5Metabolomics
- 2Liquid chromatography
- 2Metabolite identification
- 2Proteomics
- 1Acylcarnitines
-
Spring 2015
The objective of this work was to employ differential 13C/12C dansyl chloride (DnsCl) derivatization strategies for qualitative and quantitative profiling of amine- and phenol-containing metabolites by liquid chromatography electrospray ionization Fourier Transform ion cyclotron resonance mass...
-
Development and Application of Chemical Isotope Labeling Methods for Metabolomics Data Processing and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadSpring 2016
Metabolomics is the study of chemical processes involving small-molecule metabolites in a given biological system. These small molecules are downstream outputs of cellular activities. An understanding of the metabolome change can help us to visualize perturbations in the genome or proteome from...
-
Development and Applications of Mass Spectrometric Methods for Proteome Analysis and Protein Sequence Characterization
DownloadFall 2013
Mass spectrometry (MS) is widely used in proteomic work for protein identification and characterization. A key to the success of MS for protein analysis is related to sample preparation. Current sample preparation methods do not meet all the needs of protein or proteome analysis. My thesis work...
-
Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
-
Development of Liquid Chromatography Mass Spectrometry Methods for the Identification and Quantification of Acylcarnitines in Biological Samples
DownloadFall 2012
The field of metabolomics follows the Greek premise where metabolic changes are believed to be indicative of disease. Acylcarnitines, for example, can be dysregulated in the presence of various diseases including genetic metabolic disorders and multiple sclerosis. Liquid chromatography mass...
-
Development of Mass Spectrometric Methods for Membrane Proteome Analysis and Protein Sequence Mapping
DownloadSpring 2014
This thesis work mainly focused on the development of mass spectrometric methods for membrane proteome analysis and protein sequence mapping. First, the comparison of the performance of RapiGest-, PPS- and SDS-based sample preparation methods for shotgun membrane proteome analysis was...
-
Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
-
Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...