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- 7Glycerol
- 4Articular cartilage
- 4Cryopreservation
- 3Cryoprotective agents
- 2Chondrocytes
- 7Chemical and Materials Engineering, Department of
- 7Chemical and Materials Engineering, Department of/Research Articles and Materials (Chemical and Materials Engineering)
- 6Surgery, Department of
- 6Surgery, Department of/Orthopaedic Surgery
- 3Graduate and Postdoctoral Studies (GPS), Faculty of
- 3Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
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2020-01-01
Thompson, Kirsten M., Huber, Dezene P. W., Murray, Brent W.
The mountain pine beetle, Dendroctonus ponderosae (Coleoptera: Curculionidae) is a major forest pest of pines in western North America. Beetles typically undergo a one-year life cycle with larval cold hardening in preparation for overwintering. Two-year life cycle beetles have been observed but...
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Bioconversion of Lignin and Glycerol to Polyhydroxyalkanoates Using Pseudomonas putida KT2440 Under Nutrient Limited Conditions
DownloadFall 2023
Polyhydroxyalkanoates (PHAs) are a type of bioplastic synthesized by several bacterial species using various carbon sources. PHAs are biocompatible, sustainable, and biodegradable, making them attractive alternatives to traditional petroleum-based plastics. However, the price of PHAs is currently...
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2013
Yu, H., Jomha, N. M., Elliott, J. A., Al-Abbasi, K. K., McGann, L. E.
In previous research, we successfully cryopreserved intact human articular cartilage on its bone base with high chondrocyte viability using a vitrification protocol that entailed sequential exposure to several cryopreserving agents (CPAs) at lowering temperatures resulting in a high final...
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2012
Law, G. K., Forbes, J. F., Elliott, J. A., McGann, L. E., Jomha, N. M., Prasad, V., Almansoori, K. A.
Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes. However, at...
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2014
Prasad, V., Laouar, L., Jomha, N. M., McGann, L. E., Fahmy, M. D., Almansoori, K. A., Elliott, J. A.
Vitrification of articular cartilage (AC) could enhance tissue availability but requires high concentrations of cyroprotective agents (CPAs). This study investigated relative injuries caused by commonly used CPAs. We hypothesized that the in situ chondrocyte dose–injury relationships of five...
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Gene expression analysis of overwintering mountain pine beetle larvae suggests multiple systems involved in overwintering stress, cold hardiness, and preparation for spring development
Download2016
Robert, Jeanne A., Bonnett, Tiffany R., Pitt, Caitlin, Spooner, Luke J., Fraser, Jordan D., Yuen, Macaire M. S., Keeling, Christopher I., Bohlmann, Jorg, Huber, Dezene P. W.
Cold-induced mortality has historically been a key aspect of mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), population control, but little is known about the molecular basis for cold tolerance in this insect. We used RNA-seq analysis to monitor gene expression...
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IL-6 indirectly modulates the induction of glyceroneogenic enzmes in adipose tissue during exercise
Download2012
Wan, Zhongxiao, Beaudoin, Marie-Soleil, Castellani, Laura, Wright, David C., Ritchie, Ian, Chan, Catherine B.
Background Glyceroneogenesis is an important step in the control of fatty acid re-esterification with PEPCK and PDK4 being identified as key enzymes in this process. We have previously shown that glyceroneogenic enzymes such as PDK4 are rapidly induced in white adipose tissue during exercise....
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2009
This brief manuscript introduces the subject of thermodynamics and the papers of the special issue on thermodynamic aspects of cryobiology. Thermodynamic terminology is defined for the non-specialist.
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Fall 2015
Osteoarthritis, a disease resulting in the breakdown of cartilage and bone within joints, is a global burden that is growing in scope. There is no cure for osteoarthritis, and the current treatments are all lacking in some form or another. One treatment which attempts to prevent degeneration of...
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2014-09-26
Rekieh, K., Abazari, A., Elliott, J.A., Jomha, N.M., Law, G.K., McGann, L.E.
Objective: Osteochondral allografting is an effective method to treat large osteochondral defects but difficulties in tissue preservation have significantly limited the application of this technique. Successful cryopreservation of articular cartilage (AC) could improve the clinical availability...