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Skip to Search Results- 5Chen, Guanqun
- 5Weselake, Randall J.
- 4Caldo, Kristian Mark P.
- 3Greer, Michael S.
- 3Mietkiewska, Elzbieta
- 3Xu, Yang
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A 10 kDa acyl-CoA binding protein (ACBP) from Brassica napus enhances acyl exchange between acyl-CoA and phosphatidylcholine
Download2009
Yurchenko, O. P., Stymne, S., Stahl, U., Moloney, M. M., Nykiforuk, C. L., Weselake, R. J. , Banas, A.
The gene encoding a 10-kDa acyl-CoA-binding protein (ACBP) from Brassica napus wasover-expressed in developing seeds of Arabidopsis thaliana. Biochemical analysis of T2andT3A. thaliana seeds revealed a significant increase in polyunsaturated fatty acids (FAs)(18:2cisD9,12and 18:3cisD9,12,15) at...
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Broadening the genetic base of Brassica napus canola by interspecific crosses with different variants of B. oleracea
Download2018-08-01
Iftikhar, R., Wang, X., Rahman, Habibur
Broadening the genetic base of the C genome of Brassica napus canola by use of B. oleracea is important. In this study, the prospect of developing B. napus canola lines from B. napus × B. oleracea var. alboglabra, botrytis, italica and capitata crosses and the effect of backcrossing the F1’s to...
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2020-03-01
Tian, Bo, Sun, Meijuan, Jayawardana, Kethmi, Wu, Ding, Chen, Guanqun
Castor oil contains approximately 90% ricinoleic acid (RA) which is stored mainly in the form of tri‐ricinoleic acid containing triacylglycerols (TAG). Ricinoleate is synthesized from oleate (18:1n‐9) esterified to the sn‐2 position of phosphatidylcholine (PtdCho) catalyzed by...
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...
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Physaria fendleri and Ricinus communis LCAT-like phospholipases selectively cleave hydroxy acyl chains from phosphatidylcholine
Download2020-10-27
Xu, Yang, Caldo, Kristian Mark P., Singer, Stacy D., Mietkiewska, Elzbieta, Greer, Michael S., Tian, Bo, Dyer, John M., Smith, Mark, Zhou, Xue-Rong, Qiu, Xiao, Weselake, Randall J., Chen, Guanqun
Producing hydroxy fatty acids (HFAs) in transgenic crops represents a promising strategy to meet our demands for specialized plant oils with industrial applications. The expression of Ricinus communis (castor) OLEATE 12-HYDROXYLASE (RcFAH12) in Arabidopsis has resulted in only...
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The compact genome of the plant pathogen Plasmodiophora brassicae is adapted to intracellular interactions with host Brassica spp
Download2016
Clarke, Wayne E., Taheri, Ali, Rolfe, Stephen A., Malinowski, Robert, Haddadi, Parham, Links, Matthew G., Kagale, Sateesh, Strelkov, Stephen E., Djavaheri, Mohammad, Robinson, Stephen J., Parkin, Isobel A. P., Borhan, M. Hossein
Background The protist Plasmodiophora brassicae is a soil-borne pathogen of cruciferous species and the causal agent of clubroot disease of Brassicas including agriculturally important crops such as canola/rapeseed (Brassica napus). P. brassicae has remained an enigmatic plant pathogen and is a...