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- 5Weselake, Randall J.
- 4Caldo, Kristian Mark P.
- 4Xu, Yang
- 3Greer, Michael S.
- 3Mietkiewska, Elzbieta
- 24Graduate and Postdoctoral Studies (GPS), Faculty of
- 24Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 10Agricultural, Food and Nutritional Science, Department of
- 10Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Biological Sciences, Department of
- 1Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 2Campbell, Robert E. (Chemistry)
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Evaluation of oilseed Brassica napus germplasm for days to flowering under a short-day condition and QTL mapping of the trait
DownloadSpring 2021
Canola (Brassica napus, AACC = 2n = 38) is an important oilseed crop in Canada. Among the different agronomic traits, the earliness of flowering and maturity are important for growing this crop on the Canadian prairies. The earliness of flowering can be improved by using the genes/alleles capable...
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Evaluation of Spring Canola (Brassica Napus) Lines Derived from Rutabaga (Brassica Napus Var. Napobrassica) X Canola Crosses for Agronomic and Seed Quality Traits and Heterosis
DownloadSpring 2018
Brassica napus L. (AACC, 2n = 38) canola is the most extensively cultivated Brassica oilseed crop in the world; it is one of the most important field crops in Canada. The narrow genetic diversity in this crop is considered one of the major hindrances for continued improvement of this crop for...
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Genetic and molecular analysis of clubroot resistance in canola introgressed from rutabaga cvs. Polycross and Brookfield
DownloadFall 2022
Clubroot disease caused by Plasmodiophora brassicae is a serious threat to canola (Brassica napus) production. The evolution of new pathotypes has rendered available resistances ineffective and has necessitated the introgression of new resistance into canola and furthering our understanding of...
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Genetic study on Brassica rapa and Brassica napus for seed color and identification of molecular markers
DownloadFall 2012
Yellow seed in Brassica oilseed crops is favoured for its association with lower fibre and higher oil contents. Yellow-seeded Brassica napus lines have been developed through introgression of this trait from its allied species; however, the trait in B. napus is often influenced by environmental...
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...
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Improving Verticillium longisporum inoculation protocols and quantifying canola yield losses
DownloadSpring 2024
Verticillium stripe, caused by Verticillium longisporum, is an emerging soilborne disease of the Canadian canola (Brassica napus) crop. This study aimed to refine techniques for inoculating the pathogen on canola and to quantify its impact on hybrid canola yield under greenhouse and field...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...