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- 17Saccharomyces-cerevisiae
- 4DGAT
- 4Triacylglycerol
- 3Linum usitatissimum
- 2Arabidopsis-thaliana
- 5Weselake, Randall J.
- 2Caldo, Kristian Mark P.
- 2Chen, Guanqun
- 2Greer, Michael S.
- 2Judy Gnarpe
- 2Liu, Qin
- 18Graduate and Postdoctoral Studies (GPS), Faculty of
- 18Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 8Biological Sciences, Department of
- 8Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 6Agricultural, Food and Nutritional Science, Department of
- 6Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 5Department of Agricultural, Food, and Nutritional Science
- 3Department of Cell Biology
- 2Department of Biochemistry
- 2Department of Biological Sciences
- 1Department of Cell Biology and Anatomy
- 1Department of Chemical Engineering
- 1Bressler, David C (Agricultural, Food and Nutritional Science)
- 1Gänzle, Michael (Agricultural, Food and Nutritional Science)
- 1Ozga, Jocelyn (Department of Agricultural, Food and Nutritional Science)
- 1Sauvageau, Dominic (Chemical and Materials Engineering)
- 1Stuart, David (Biochemistry)
- 1Taylor, Gregory (Biological Sciences)
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Evolution of sourdough microbiota in spontaneous sourdoughs started with different plant materials
Download2016-01-01
Ripari, Valery, Gänzle, Michael G., Berardi, Enrico
The preparation of sourdough in bakeries may include the use of inocula, e.g. fruits, flowers or rumen cuts to accelerate the process of selection of suitable microorganisms. The aim of this work was to investigate the effect of these inocula on the microbial evolution in sourdoughs. First, the...
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Exploring the role of the thioredoxin system, peroxiredoxins and glutaredoxins in aluminum and cadmium tolerance in yeast and Arabidopsis thaliana
DownloadFall 2011
Aluminum (Al) and cadmium (Cd) are non-redox active metal ions of agricultural importance. Both are able to induce oxidative damage as a mechanism of toxicity. To increase our understanding of the mechanisms of Al and Cd toxicity and tolerance in plants, the potential role of antioxidant enzymes...
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Functional and Topological Analysis of Acyl-CoA:Diacylglycerol Acyltransferase 2 From Saccharomyces cerevisiae
DownloadSpring 2011
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20, DGAT or DAGAT) is a membrane protein found mainly in the endoplasmic reticulum (ER). It catalyzes the final step in the biosynthesis of triacylglyerol (TAG or TG), which is the principal repository of fatty acids for energy utilization and...
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Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis
Download2011
Siloto, Rodrigo M. P., Snyder, Crystal L., Liu, Qin, Weselake, Randall J.
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...
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Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
Download2007
Pozniakovsky, A., Hyman, A.A., Stirling, P.C., Takhar, K.S., Srayko, M., Lerou, M.R.
The Chaperonin Containing Tcp1 (CCT) maintains cellular protein folding homeostasis in the eukaryotic cytosol by assisting the biogenesis of many proteins, including actins, tubulins, and regulators of the cell cycle. Here, we demonstrate that the essential and conserved eukaryotic phosducin-like...
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Gene coexpression clusters and putative regulatory elements underlying seed storage reserve accumulation in Arabidopsis
Download2011
Peng, Fred Y., Weselake, Randall J.
Background In Arabidopsis, a large number of genes involved in the accumulation of seed storage reserves during seed development have been characterized, but the relationship of gene expression and regulation underlying this physiological process remains poorly understood. A more holistic view of...
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Heterologous expression of flax PHOSPHOLIPID:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE (PDCT) increases polyunsaturated fatty acid content in yeast and Arabidopsis seeds
Download2015
Weselake, Randall J., Mietkiewska, Elzbieta, Pan, Xue, Singer, Stacy D., Siloto, Rodrigo M. P., Wickramarathna, Aruna D.
Background Flax (Linum usitatissimum L.) is an agriculturally important crop with seed oil enriched in α-linolenic acid (18:3 cisΔ9, 12, 15; ALA). This polyunsaturated fatty acid (PUFA) is the major determinant for the quality of flax seed oil in food, nutraceuticals and industrial applications....
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How studying the interaction of Sir4 with a subset of Nups uncovered a new chromatin bound complex.
DownloadFall 2017
The nucleus is the defining feature of eukaryotic cells and its major function is to house the cell’s genome and segregate it from the surrounding cytoplasm. The membranes encapsulating the nucleus constitute the nuclear envelope (NE). The interactions between the NE and chromatin facilitate the...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...