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- 5Department of Chemistry
- 4Department of Electrical and Computer Engineering
- 3Department of Agricultural, Food, and Nutritional Science
- 1Department of Agricultural, Food and Nutritional Science
- 1Department of Biochemistry
- 1Department of Medical Microbiology and Infectious Diseases
- 2Kurgan, Lukasz (Electrical and Computer Engineering)
- 1Ball, Ronald O. (Department of Agricultural, Food and Nutritional Science)
- 1Ballermann, Barbara (Medicine)
- 1Boekhoven, Job (Chemistry, Technical University of Munich)
- 1D. Jed Harrison (Chemistry)
- 1Dr. Jianping Wu, Department of Agricultural,Food and Nutritional Sciences
Extraction of canola proteins and assessment of their applications: cruciferin for encapsulation of bioactives and napin as a chaperone-like moleculeDownload
Canola is a farm-gate crop in Canada. Canola meal after oil extraction is used mostly as animal feed with limited value-added applications. Canola proteins are known to have great potential for use in food and non-food applications due to their nutritional, biological and functional properties....
Simultaneous measurement of protein and energy metabolism and application to determine lysine requirements in sowsDownload
Simultaneous measurements of energy and protein metabolism can provide valuable information about their interactions. Dietary lysine is limiting in typical feedstuffs fed to swine and, therefore, limits protein synthesis. Current recommendations for dietary amino acid and energy intakes may not...
The effects of broiler breeder dietary energy and protein during rearing and dietary energy level of lay diets on egg composition, offspring performance and carcass yield were evaluated. Pullets were fed a diet containing high ME (2,736 kcal/kg; HEREAR), or low ME (2,528 kcal/kg; LEREAR) combined...
The development of rapid and bioorthogonal chemical reactions has expanded greatly the utility of bioconjugation chemistry in the service of site-selective protein labeling, even allowing molecular imaging in live cells or animals. âClickâ chemistry is particularly desirable due to its fast...
An examination of linking and blocking procedures for use in deflection cantilever array-based protein detectionDownload
In this project common linking and blocking procedures were examined for use in multiplexed deflection cantilever array-based detection of proteins. A human interferon gamma (INF-γ) enzyme-linked immunosorbent assay (ELISA) kit was used to evaluate the effectiveness of the linking and blocking...
This dissertation presents the preparation of functional bioinorganic hybrids from silicon-based quantum dots (SiQDs) and enzymes and the use of SiQDs and green fluorescent protein as ratiometric photoluminescent sensors for nerve agents. Chapter 2 discusses the preparation of water-soluble...
Development and Application of Protected Amino Acids for the Installation of Stable Post-translational Modifications Using Solid-Phase Peptide SynthesisDownload
This thesis describes the synthesis of non-canonical amino acid building blocks and their use in solid-phase peptide synthesis (SPPS) to explore peptidic drug analogues based on natural products. We replaced sensitive functionalities found in the structure of naturally produced peptides with...
Structure and dynamics of biomolecules: probing muscle regulation, prion protein unfolding, and drug insertion into DNA by nuclear magnetic resonance spectroscopyDownload
Nuclear magnetic resonance (NMR) spectroscopy is a powerful approach to study the structure and dynamics of macromolecules in a close-to-native solution environment. In the present thesis I present my investigation of protein and nucleic acid structure and dynamics in a wide variety of biological...
Computational support systems for prediction and characterization of protein crystallization outcomesDownload
Analysis of protein structures may reveal their function, regulation and interactions. Almost 90% of the known protein structures were solved using X-ray crystallography; however, many more structures remain unsolved. Protein Structure Initiative (PSI) project was created to speed up structure...