Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins

  • Author / Creator
    Cao, Cong
  • Cellulose nanocrystals (CNCs) are an emerging renewable and sustainable
    nanomaterial which has received increasing attention. They are abundant, nontoxic,
    biodegradable and chemically inert. CNCs have unique characteristics not
    met by traditional cellulose-derived materials, leading to
    investigations/applications of CNC's across all disciplines. The objective of this
    work is to investigate interactions of proteins with CNC's by atomic force
    microscopy (AFM) and spectroscopic techniques. Results indicated an
    electrostatic driven interaction that depends on incubation time, protein
    concentration and protein intrinsic properties such as molecular weight, charge
    distribution and iso-electric point. Understanding CNC-protein interactions aids in
    the development of new biosensing platforms that utilize green-material substrates.
    The usefulness of a CNC surface as a substrate for immunoassays was developed
    and evaluated by surface plasmon resonance (SPR). CNC films were shown to
    provide a suitable platform for antibody immobilization and antigen binding, with
    LOD’s comparable to other SPR based biosensors.

  • Subjects / Keywords
  • Graduation date
    Fall 2013
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Serpe, Michael J. (Chemistry)
    • Lucy, Charles A. (Chemistry)