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Investigating Interactions between Cellulose Nanocrystals (CNCs) and Proteins

  • Author / Creator
    Cao, Cong
  • Cellulose nanocrystals (CNCs) are an emerging renewable and sustainable nanomaterial which has received increasing attention. They are abundant, nontoxic, biodegradable and chemically inert. CNCs have unique characteristics not met by traditional cellulose-derived materials, leading to investigations/applications of CNC's across all disciplines. The objective of this work is to investigate interactions of proteins with CNC's by atomic force microscopy (AFM) and spectroscopic techniques. Results indicated an electrostatic driven interaction that depends on incubation time, protein concentration and protein intrinsic properties such as molecular weight, charge distribution and iso-electric point. Understanding CNC-protein interactions aids in the development of new biosensing platforms that utilize green-material substrates. The usefulness of a CNC surface as a substrate for immunoassays was developed and evaluated by surface plasmon resonance (SPR). CNC films were shown to provide a suitable platform for antibody immobilization and antigen binding, with LOD’s comparable to other SPR based biosensors.

  • Subjects / Keywords
  • Graduation date
    2013-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3CD6P
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Chemistry
  • Supervisor / co-supervisor and their department(s)
    • McDermott, Mark T. (Chemistry)
  • Examining committee members and their departments
    • Serpe, Michael J. (Chemistry)
    • Lucy, Charles A. (Chemistry)