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Skip to Search Results- 10Gänzle, Michael G.
- 2Garcia-Hernandez, Rigoberto
- 2McMullen, Lynn
- 2McMullen, Lynn M.
- 1Aalhus, Jennifer L.
- 1Balay, Danielle
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Comparative assessment of qPCR enumeration methods that discriminate between live and dead Escherichia coli O157:H7 on beef
Download2018-01-01
Laidlaw, Anna M., Gänzle, Michael G., Yang, Xianqin
Quantitative Polymerase Chain Reaction (qPCR) is a molecular method commonly used to detect and quantify bacterial DNA on food but is limited by its inability to distinguish between live and dead cell DNA. To overcome this obstacle, propidium monoazide (PMA) alone or with deoxycholate (DC) was...
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Detection of enterohaemorrhagic Escherichia coli in food by droplet digital PCR to detect simultaneous virulence factors in a single genome
Download2020-09-01
He, Li, Simpson, David, Gänzle, Michael G.
Shiga toxin producing E. coli are a problem for food producers. STEC's require a combination of virulence factors to cause disease, so ideally detection techniques should detect the presence of multiple virulence factors in a single cell directly from food. Droplet Digital PCR (ddPCR) is commonly...
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Development and validation of a surrogate strain cocktail to evaluate bactericidal effects of pressure on verotoxigenic Escherichia coli
Download2015-01-01
Garcia-Hernandez, Rigoberto, McMullen, Lynn, Gänzle, Michael G.
Many strains of verotoxigenic Escherichia coli (VTEC) are highly resistant to pressure. To facilitate future studies to improve the elimination of VTEC by pressure processing of food, this study developed and validated a cocktail of non-pathogenic strains of E. coli with equal or higher...
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Effect of chitosan, and bacteriocin – Producing Carnobacterium maltaromaticum on survival of Escherichia coli and Salmonella Typhimurium on beef
Download2018-01-01
Hu, Zi Yi, Balay, Danielle, Hu, Ying, McMullen, Lynn M., Gänzle, Michael G.
The aim of this study was to investigate the synergistic effect of chitosan and bacteriocins against Escherichia coli and Salmonella in media and in lean beef. The inhibitory effects of chitosan and bacteriocins against E. coli AW1.7 and S. enterica Typhimurium in media were determined by a...
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Effect of drying on oxidation of membrane lipids and expression of genes encoded by the Shiga toxin prophage in Escherichia coli
Download2020-01-01
Fang, Yuan, McMullen, Lynn M., Gänzle, Michael G.
Drying processes do not eliminate pathogenic Escherichia coli in foods but induce sublethal injury, which may also induce the Shiga toxin (Stx) prophage. This study investigated the effect of drying on membrane lipid oxidation and stx expression in E. coli. Lipid peroxidation was probed with...
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Effect of hydrostatic pressure and antimicrobial on survival of Listeria monocytogenes and enterohaemorrhagic E.coli in beef
Download2016-01-01
The effects of marinades on Listeria monocytogenes and enterohaemorrhagic Escherichia coli were investigated in pressure treated beef steaks. Meat was treated with 600 MPa or 450 MPa. Marinades did not enhance pressure inactivation of E. coli in beef steaks and marinades prevented...
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Effect of sodium chloride and chitosan on the inactivation of heat resistant or Shiga-toxin producing Escherichia coli during grilling of burger patties
Download2019-01-01
Hu, Ziyi, Rohde, Alina, McMullen, Lynn, Gänzle, Michael G.
Cattle are a reservoir for enterohemorrhagic Escherichia coli (EHEC), and ground beef is a major vehicle for human infection with EHEC. Heat resistance of E. coli, including EHEC, is impacted by NaCl and other additives. This study aimed to evaluate the effect of NaCl and other additives on the...
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2015
Garcia-Hernandez, Rigoberto, Ruan, Lifang, Gänzle, Michael G., Zheng, Jinshui, Mercer, Ryan G., McMullen, Lynn M.
Escherichia coli AW1.7 is a heat resistant food isolate and the occurrence of pathogenic strains with comparable heat resistance may pose a risk to food safety. To identify the genetic determinants of heat resistance, 29 strains of E. coli that differed in their of heat resistance were analyzed...
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Improving beef hamburger quality and fatty acid profiles through dietary manipulation and exploitation of fat depot heterogeneity
Download2014
Dugan, Michael E. R., Rolland, David C., Uttaro, Bethany, Aalhus, Jennifer L., Block, Hushton C., Vahmani, Payam, Mapiye, Cletos, Proctor, Spencer D., McAllister, Timothy A.
Background Hamburger is the most consumed beef product in North America, but lacks in nutritional appeal due to its high fat content and high proportion of saturated fatty acids (SFA). Objectives of the present study were to improve the FA profiles of hamburgers made with perirenal fat (PRF) and...
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Influence of cyclopropane fatty acids on heat, high pressure, acid, and oxidative stress resistance in Escherichia coli
Download2016-01-01
Chen, Yuan Yao, Gänzle, Michael G.
Heat and high pressure resistant strains of Escherichia coli are a challenge to food safety. This study investigated effects of cyclopropane fatty acids (CFAs) on stress tolerance in the heat- and pressure-resistant strain E. coli AW1.7 and the sensitive strain E. coli MG1655. The role of CFAs...