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Skip to Search Results- 22Graduate and Postdoctoral Studies (GPS), Faculty of
- 22Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 18Chemistry, Department of
- 18Chemistry, Department of/Journal Articles (Chemistry)
- 13Biological Sciences, Department of
- 13Biological Sciences, Department of/Journal Articles (Biological Sciences)
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
Download2009
Sun, Nian, Klassen, John S., Kitova, Elena N., Sun, Jiangxiao
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
2009
Kitova, Elena N., Klassen, John S., Sun, Nian, Sun, Jiangxiao
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Impact of Farming Practices on Soil Bacterial Community Composition, Diversity, and Interactions in the Main Agricultural Regions of Alberta
DownloadSpring 2022
Soil health refers to “the capacity of soil to function as a vital living system”, implying not only the capacity of soil to providing services of human interest, but also its capacity to provide ecological services, which makes ecosystems sustainable for the long term. The assessment of soil...
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Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera
Download2020-02-01
Wong, Barbara, Leal, Isabel, Feau, Nicolas, Dale, Angela, Uzunovic, Adnan, Hamelin, Richard C.
Wood and wood products can harbor microorganisms that can raise phytosanitary concerns in countries importing or exporting these products. To evaluate the efficacy of wood treatment on the survival of microorganisms of phytosanitary concern the method of choice is to grow microbes in petri dishes...
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Molecular cloning of cDNA encoding a 55-kDa multifunctional thyroid hormone binding protein of skeletal muscle sarcoplasmic reticulum
Download1990-01-01
Fliegel, Larry, Newton, E., Burns, K., Michalak, M.
A cDNA clone encoding 55-kDa multifunctional, thyroid hormone binding protein of rabbit skeletal muscle sarcoplasmic reticulum was isolated and sequenced. The cDNA encoded a protein of 509 amino acids, and a comparison of the deduced amino acid sequence with the NH2-terminal amino acid sequence...
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Molecular Dynamics Simulations of Polyethylenimine Mediated Nucleic Acid Complexation with Implications for Non-viral Gene Delivery
DownloadSpring 2013
Gene therapy is a promising therapeutic technique that involves delivering nucleic acids into cells. Polycations have evolved into a major category of gene carriers. Polyethylenimine (PEI) is one of the most effective polycationic carriers. Furthermore, modifying PEI with certain lipophilic...
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Spring 2016
Hybrids formed by biological entities and human-made nano-structures have been intensively studied in recent years due to their very interesting properties and applications. DNA-carbon nanotube (CNT) hybrid is one such material and the motivation of this PhD study. The interaction of DNA building...
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Nonspecific interactions between proteins and charged biomolecules in electrospray ionization mass spectrometry
Download2010
Soya, Naoto, Klassen, John S., Kitova, Elena N., Sun, Nian
An investigation of the nonspecific association of small charged biomolecules and proteins in electrospray ionization mass spectrometry (ES-MS) is described. Aqueous solutions containing pairs of proteins and a small acidic or basic biomolecule that does not interact specifically with either of...