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A simple procedure to secure an indwelling jugular vein catheter to the neck of cattle for repeated blood sampling
Download2001
MacLean, Melissa A., Zalkovic, Pavol, Ambrose, Divakar J.
Abstract A procedure to secure a jugular vein catheter system to the neck of cattle is described. A wide-mouthed polypropylene bottle attached to a halter strap provided a secure container for the catheter. This device allowed convenient access to the catheter for repeated sampling and,...
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2013-01-01
Basu, Urmila, Josue, Romao Moura, Guan, LeLuo
The recent technological innovations in the area of functional genomics, gene expression/transcriptomic profiling can provide new insights to understand the molecular basis of adipogenesis. The focus of this review is to highlight the recent advances in our understanding of the complex interplay...
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Altered microRNA expression in bovine subcutaneous and visceral adipose tissues from cattle under different diet
Download2012
Guan, Leluo, Romao, Josue M., McAllister, Tim, He, Maolong, Jin, Weiwu
Background MicroRNAs (miRNAs) are a class of molecular regulators found to participate in numerous biological processes, including adipogenesis in mammals. This study aimed to evaluate the differences of miRNA expression between bovine subcutaneous (backfat) and visceral fat depots (perirenal...
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Characterization of the altered gene expression profile in early porcine embryos generated from parthenogenesis and somatic cell chromatin transfer
Download2014
Dobrinsky, John, Zhou, Chi, Dixon, Walter T., Dyck, Michael K., Stothard, Paul, Foxcroft, George R., Tsoi, Stephen, Verstegen, John
The in vitro production of early porcine embryos is of particular scientific and economic interest. In general, embryos produced from in vitro Assisted Reproductive Technologies (ART) manipulations, such as somatic cell chromatin transfer (CT) and parthenogenetic activation (PA), are less...
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Diacylglycerol acyltransferase (DGAT) activity in relation to muscle fat content and DGAT1 genotype in two different breeds of Bos taurus
Download2006-01-01
Sorensen, Brent M., Kühn, Christa, Teuscher, Friedrich, Schneider, Falk, Weselake, Randall J., Wegner, Jochen J.
To circumvent the obvious waste of resources when producing good marbled beef, research is needed to clarify mechanisms which are involved in intramuscular fat storage in cattle. The possible association between the activity of diacylglycerol acyltransferase (DGAT) and muscle fat content was...
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Elucidation of molecular mechanisms of physiological variations between bovine subcutaneous and visceral fat depots under different nutritional regimes
Download2013
Jin, Weiwu, He, Maolong, Guan, Leluo, Romao, Josue M., McAllister, Tim
Adipose tissue plays a critical role in energy homeostasis and metabolism. There is sparse understanding of the molecular regulation at the protein level of bovine adipose tissues, especially within different fat depots under different nutritional regimes. The objective of this study was to...
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2012
Wang, Lusheng, Moore, Steve S., Cai, Zhipeng, Goebel, Randy, Lin, Guohui, Wang, Yining, Stothard, Paul
Background Single nucleotide polymorphism (SNP) genotyping assays normally give rise to certain percents of no-calls; the problem becomes severe when the target organisms, such as cattle, do not have a high resolution genomic sequence. Missing SNP genotypes, when related to target traits, would...
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Genome wide identification and functional prediction of long non-coding RNAs responsive to Sclerotinia sclerotiorum infection in Brassica napus
Download2016
Rahman, Muhammad H., Megha, Swati, Joshi, Raj K., Basu, Urmila, Kav, Nat N. V.
Sclerotinia stem rot caused by Sclerotinia sclerotiorum affects canola production worldwide. Emerging evidence suggests that long non-coding RNAs (lncRNAs) play important roles in the regulation of gene expression in plants, in response to both abiotic and biotic stress. So far, identification of...
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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2012
Wan, Zhongxiao, MacDonald, Tara, Wright, David C., Holloway, Graham P., Chan, Catherine B., Perry, Christopher G.
Background Adipose tissue mitochondria have been implicated as key mediators of systemic metabolism. We have shown that IL-6 activates AMPK, a mediator of mitochondrial biogenesis, in adipose tissue; however, IL-6−/− mice fed a high fat diet have been reported to develop insulin resistance. These...