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- 10Xu, Yang
- 8Weselake, Randall J.
- 6Pan, Xue
- 5Caldo, Kristian Mark P.
- 5Mietkiewska, Elzbieta
- 38Graduate and Postdoctoral Studies (GPS), Faculty of
- 38Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 16Agricultural, Food and Nutritional Science, Department of
- 16Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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- 6Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 21Department of Agricultural, Food, and Nutritional Science
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- 2Department of Agricultural, Food and Nutritional Science
- 1Department of Biochemistry
- 1Department of Botany
- 1Department of Chemical Engineering
- 3Deyholos, Michael (Biological Sciences)
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Allen Good (Biological Sciences)
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Characterization of a Type‐2 Diacylglycerol Acyltransferase from Haematococcus pluvialis Reveals Possible Allostery of the Recombinant Enzyme
Download2019-12-26
Nguyen, Trinh, Xu, Yang, Abdel-Hameed, Mona, Sorensen, John L., Singer, Stacy D., Chen, Guanqun
Haematococcus pluvialis is a green microalga used in the algal biotechnology industry that can accumulate considerable amounts of storage triacylglycerol (TAG) and astaxanthin, which is a high‐value carotenoid with strong antioxidant activity, under stress conditions. Diacylglycerol...
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Fall 2013
Lectins are proteins that bind specifically to carbohydrates and are active in plant defense and other processes. The sequencing of the flax (Linum usitatissimum) genome showed that it was unusually rich in genes predicted to encode one or more agglutinin domains (PFAM PF07468). This domain is...
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Characterization of Type-2 Diacylglycerol Acyltransferases in the Green Microalga Chromochloris zofingiensis
Download2018-12-13
Xu, Yang, Falarz, Lucas, Chen, Guanqun
Diacylglycerol acyltransferase (DGAT) catalyzes the last and committed step of the acyl-CoA-dependent TAG biosynthesis and thus is a key target for manipulating oil production in microalgae. The microalga Chromochloris zofingiensis can accumulate substantial amounts of triacylglycerol (TAG) and...
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Comparative transcriptome analysis of rutabaga (Brassica napus) cultivars in response to Plasmodiophora brassicae
DownloadFall 2020
Clubroot, a soil-borne disease caused by the obligate parasite Plasmodiophora brassicae Woronin, is a threat to canola (Brassica napus L.) production in western Canada. Genetic resistance represents the most effective tool to manage this disease. To improve understanding of the mechanisms of...
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Spring 2014
Metabolomics involves the high throughput characterization of small molecules or metabolites in cells, tissues and organisms. To interpret, store and exchange metabolomic data it is necessary to have comprehensive, electronically accessible databases that can be used to handle both the...
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Evaluation of oilseed Brassica napus germplasm for days to flowering under a short-day condition and QTL mapping of the trait
DownloadSpring 2021
Canola (Brassica napus, AACC = 2n = 38) is an important oilseed crop in Canada. Among the different agronomic traits, the earliness of flowering and maturity are important for growing this crop on the Canadian prairies. The earliness of flowering can be improved by using the genes/alleles capable...
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Evaluation of Spring Canola (Brassica Napus) Lines Derived from Rutabaga (Brassica Napus Var. Napobrassica) X Canola Crosses for Agronomic and Seed Quality Traits and Heterosis
DownloadSpring 2018
Brassica napus L. (AACC, 2n = 38) canola is the most extensively cultivated Brassica oilseed crop in the world; it is one of the most important field crops in Canada. The narrow genetic diversity in this crop is considered one of the major hindrances for continued improvement of this crop for...
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Evolutionary and biochemical characterization of a Chromochloris zofingiensis MBOAT with wax synthase and DGAT activity
Download2021-01-01
Xu, Yang, Pan, Xue, Lu, Junhao, Wang, Juli, Shan, Qiyuan, Stout, Jake, Chen, Guanqun
Abstract: Wax synthase (WS) catalyzes the last step in wax ester biosynthesis in green plants. Two unrelated subfamilies of WS including the bifunctional acyltransferase and plant-like WS have been reported, but the latter is largely uncharacterized in microalgae. Here, we functionally...
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Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis
Download2011
Siloto, Rodrigo M. P., Snyder, Crystal L., Liu, Qin, Weselake, Randall J.
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...