Search
Skip to Search Results-
MicroRNA Buffering and Altered Variance of Gene Expression in Response to Salmonella Infection
Download2014
Plastow, G. S., Bao, H., Kommadath, A., Guan, L. L., Tuggle, C. K., Stothard, P.
One potential role of miRNAs is to buffer variation in gene expression, although conflicting results have been reported. To investigate the buffering role of miRNAs in response to Salmonella infection in pigs, we sequenced miRNA and mRNA in whole blood from 15 pig samples before and after...
-
1988
Plastow, G. S., Mileham, A. J., Siggens, K. W.
A 3.8 kb male specific fragment was observed following agarose gel etectrophoresis of porcine DNA digested with Sph I. DNA was recovered from this region of a gel using DE-81 paper and ligated to Sph I digested pUC18. Plasmids carrying male specific sequences were identified by their differential...
-
Increasing gene discovery and coverage using RNA-seq of globin RNA reduced porcine blood samples
Download2014
Stothard, P., Fritz-Waters, E., Choi, I., Abrams, S. M., Kommadath, A., Hosseini, A., Plastow, G. S., Guan, L. L., Tuggle, C. K., Bao, H., Meng, Y., Lunney, J. K., Reecy, J. M., Sun, X.
Background Transcriptome analysis of porcine whole blood has several applications, which include deciphering genetic mechanisms for host responses to viral infection and vaccination. The abundance of alpha- and beta-globin transcripts in blood, however, impedes the ability to cost-effectively...
-
Identification of a putative quantitative trait nucleotide in guanylate binding protein 5 for host response to PRRS virus infection
Download2015
Choi, I., Guan, L., Plastow, G. S., Liu, P., Koltes, J. E., Loyd, H., Boddicker, N. J., Eisley, C. J., Schroyen, M., Bao, H., Dekkers, J. C. M., Kommadath, A., Abrams, S. M., Reecy, J. M., Rowland, R. R. R., Serão, N. V. L., Lunney, J. K., Carpenter, S., Stothard, P., Fritz-Waters, E., Tuggle, C. K.
Background Previously, we identified a major quantitative trait locus (QTL) for host response to Porcine Respiratory and Reproductive Syndrome virus (PRRSV) infection in high linkage disequilibrium (LD) with SNP rs80800372 on Sus scrofa chromosome 4 (SSC4). Results Within this QTL,...
-
Genome-wide association studies (GWAS) identify a QTL close to PRKAG3 affecting meat pH and colour in crossbred commercial pigs
Download2015
Miar, Y., Plastow, G. S., Charagu, P., Wang, Z., Kemp, R. A., Bruce, H., Zhang, Z., Yang, T.
Background Improving meat quality is a high priority for the pork industry to satisfy consumers’ preferences. GWAS have become a state-of-the-art approach to genetically improve economically important traits. However, GWAS focused on pork quality are still relatively rare. Results Six...
-
2001
Ciobanu, D. C., Nagy, A., Day, A. E., Plastow, G. S., Rothschild, M. F., Wales, R.
Analysis of the genetic variation of an endangered population is an important component for the success of conservation. Animals from two local Romanian pig breeds, the Mangalitsa and Bazna, were analysed for variation at a number of genetic loci using PCR-based DNA tests. Polymorphism was...
-
Gene co-expression network analysis identifies porcine genes associated with variation in Salmonella shedding
Download2014
Plastow, G. S., Bearson, S. M. D., Arantes, A. S., Bao, H., Kommadath, A., Stothard, P., Guan, L. L., Tuggle, C. K.
Background Salmonella enterica serovar Typhimurium is a gram-negative bacterium that can colonise the gut of humans and several species of food producing farm animals to cause enteric or septicaemic salmonellosis. While many studies have looked into the host genetic response to Salmonella...
-
Expansion of ruminant-specific microRNAs shapes target gene expression divergence between ruminant and non-ruminant species
Download2013
Kommadath, A., Bao, H., Meng, Y., Plastow, G. S., Stothard, P., Sun, X., Arantes, A. S., Guan, L. L.
Background Understanding how species-specific microRNAs (miRNAs) contribute to species-specific phenotypes is a central topic in biology. This study aimed to elucidate the role of ruminant-specific miRNAs in shaping mRNA expression divergence between ruminant and non-ruminant species. ...
-
Estimation of genetic and phenotypic parameters for ultrasound and carcass merit traits in crossbred beef cattle
Download2014
Bruce, H. L., Plastow, G. S., Moore, S. S., Wang, Z., Nkrumah, D., Miar, Y., Durunna, O.
Ultrasound measurements of 852 crossbred steers along with carcass merit measurements on 756 of them were used to examine their genetic and phenotypic parameters. Traits including ultrasound backfat thickness (UBF), ultrasound ribeye area (UREA), ultrasound marbling (UMAR), carcass weight (CWT),...
-
Development of carcass cuts, organs, body tissue and chemical body composition during growth of pigs
Download2006
Landgraf, S., Roehe, R., Plastow, G. S., Kalm, E., Looft, H., Susenbeth, A., Knap, P. W.
A serial slaughter trial was carried out to examine the developmental change of physical and chemical body composition in pigs highly selected for lean content. A total of 48 pigs (17 females and 31 castrated males) were serially slaughtered and chemically analysed. Eight pigs were slaughtered at...