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Skip to Search Results- 3Cryoprotective agents
- 3Vitrification
- 2Articular cartilage
- 2Cryopreservation
- 1Chondrocytes
- 1Computer modeling
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2013
Yu, H., Jomha, N. M., Elliott, J. A., Al-Abbasi, K. K., McGann, L. E.
In previous research, we successfully cryopreserved intact human articular cartilage on its bone base with high chondrocyte viability using a vitrification protocol that entailed sequential exposure to several cryopreserving agents (CPAs) at lowering temperatures resulting in a high final...
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2014
Prasad, V., Laouar, L., Jomha, N. M., McGann, L. E., Fahmy, M. D., Almansoori, K. A., Elliott, J. A.
Vitrification of articular cartilage (AC) could enhance tissue availability but requires high concentrations of cyroprotective agents (CPAs). This study investigated relative injuries caused by commonly used CPAs. We hypothesized that the in situ chondrocyte dose–injury relationships of five...
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Investigating cryoinjury using simulations and experiments: 1. TF-1 cells during two-step freezing (rapid cooling interrupted with a hold time)
Download2014-09-22
McGann, L. E., Ross-Rodriguez, L. U., Elliott, J. A.
There is significant interest in designing a cryopreservation protocol for hematopoietic stem cells (HSC) which does not rely on dimethyl sulfoxide (Me2SO) as a cryoprotectant. Computer simulations that describe cellular osmotic responses during cooling and warming can be used to optimize the...
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Statistical prediction of the vitrifiability and glass stability of multi-component cryoprotective agent solutions.
Download2010
Forbes, J. F., Law, G. K., Weiss, A. D., Scheuerman, A., McGann, L. E., Elliott, J. A., Jomha, N. M.
Long-term biologic storage of articular cartilage has proven elusive due to cellular degradation over time or acute damage during attempts at cryopreservation. Vitrification is one option that may result in successful cryopreservation but difficulty with cryoprotective agent (CPA) toxicity at...