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- 1Whitford, Denise Samantha
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Coming in from the cold: spatiotemporal regulation of gene expression by the cyanobacterial RNA helicase redox (CrhR) during abiotic stress - Supplementary Data
Coming in from the cold: spatiotemporal regulation of gene expression by the cyanobacterial RNA helicase redox (CrhR) during abiotic stress - Supplementary Data
Download2024-05-10
Contains supplementary protein quantification files and cell imaging movies related to the thesis Coming in from the cold: spatiotemporal regulation of gene expression by the cyanobacterial RNA helicase redox (CrhR) during abiotic stress, Brendan T. Whitman, Spring 2024
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Fall 2012
The DEAD-box cyanobacterial RNA helicase redox, or CrhR, in Synechocystis sp. PCC 6803 is capable of unwinding dsRNA and in annealing ssRNA in a bidirectional ATP-dependent manner. This is a feature shared by only four other DEAD-box RNA helicases. Two of which, the eukaryotic p68 and p72...
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Fall 2020
RNA helicases function in all aspects of RNA metabolism, regulating a diverse range of cellular processes by rearranging RNA structures and mediating RNA-protein interactions. Prokaryotic RNA helicases have been shown to have roles in RNA degradation, ribosome biogenesis and translation, with...
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Fall 2013
West Nile virus (WNV) is a neurotropic, blood-borne flavivirus that can cause serious neurological disease in humans and animals. While significant progress has been made in identifying virus-encoded pathogenic determinants, very little is known regarding how these viral proteins interact with...
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Investigation of mRNP export kinetics and gene expression regulation by the DEAD-box protein Dbp5p in Saccharomyces cerevisiae
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The intricate architecture of the eukaryotic cell, which includes the partitioning of the cell into discrete membrane bound organelles, necessitates that various cargos must cross the nuclear envelope (NE). Nuclear pore complexes (NPCs) are large macromolecular assemblies embedded within the NE...
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2012-06-21
Whitford, Denise S., Owttrim, George W.
Expression of the stress-regulated RNA helicase of Synechocystis sp. PCC 6803, CrhR, is altered by three known stresses, temperature, light and salt concentration. Here we show that altering the source of fixed nitrogen, either urea, ammonia, nitrate or lack of fixed nitrogen, also results in...
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Proteolysis autoregulates expression of the cyanobacterial DEAD-box RNA helicase CrhR, in response to change in growth temperature
DownloadFall 2013
The DEAD box cyanobacterial RNA helicase redox, CrhR, in Synechocystis sp. PCC 6803 is induced in response to environmental stress conditions causing reduction of the redox status of the photosynthetic electron transport chain, with temperature being one of the major conditions commonly...