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- 22Carbohydrates
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- 28Graduate and Postdoctoral Studies (GPS), Faculty of
- 28Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 15Chemistry, Department of
- 15Chemistry, Department of/Journal Articles (Chemistry)
- 12Biological Sciences, Department of
- 12Biological Sciences, Department of/Journal Articles (Biological Sciences)
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Screening carbohydrate libraries for protein interactions using the direct ESI-MS assay: Applications to libraries of unknown concentration
Download2014
El-Hawiet, Amr, Klassen, John S., Kitova, Elena N.
A semiquantitative electrospray ionization mass spectrometry (ESI-MS) binding assay suitable for analyzing mixtures of oligosaccharides, at unknown concentrations, for interactions with target proteins is described. The assay relies on the differences in the ratio of the relative abundances of...
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Simultaneous measurement of protein and energy metabolism and application to determine lysine requirements in sows
DownloadSpring 2011
Simultaneous measurements of energy and protein metabolism can provide valuable information about their interactions. Dietary lysine is limiting in typical feedstuffs fed to swine and, therefore, limits protein synthesis. Current recommendations for dietary amino acid and energy intakes may not...
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Spring 2014
Two studies examined the effects of 3 commercial cat diets varying in glycemic index (GI) based on ingredient composition and starch content (34.1, 29.5, and 23.6% NFE for high, medium, and low GI, respectively) on metabolizable energy (ME), indirect calorimetry and blood measurements. In Study...
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Structure and dynamics of biomolecules: probing muscle regulation, prion protein unfolding, and drug insertion into DNA by nuclear magnetic resonance spectroscopy
DownloadSpring 2011
Nuclear magnetic resonance (NMR) spectroscopy is a powerful approach to study the structure and dynamics of macromolecules in a close-to-native solution environment. In the present thesis I present my investigation of protein and nucleic acid structure and dynamics in a wide variety of biological...
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Structure and stability of carbohydrate-lipid interactions: Methylmannose polysaccharide-fatty acid complexes
Download2016
Klassen, John S., Siuda, Iwona, Renaud, Justin, Tieleman, D. Peter, Kitova, Elena N., Liu, Lan, Mayer, Paul M., Richards, Michele R., Lowary, Todd L.
We report a detailed study of the structure and stability of carbohydrate–lipid interactions. Complexes of a methylmannose polysaccharide (MMP) derivative and fatty acids (FAs) served as model systems. The dependence of solution affinities and gas-phase dissociation activation energies (Ea) on FA...
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Structure and stability of carbohydrate-lipid interactions: Methylmannose polysaccharide-fatty acid complexes
Download2016
Mayer, Paul M., Richards, Michele R., Lowary, Todd L., Renaud, Justin, Klassen, John S., Tieleman, D. Peter, Kitova, Elena N., Liu, Lan, Siuda, Iwona
We report a detailed study of the structure and stability of carbohydrate–lipid interactions. Complexes of a methylmannose polysaccharide (MMP) derivative and fatty acids (FAs) served as model systems. The dependence of solution affinities and gas-phase dissociation activation energies (Ea) on FA...
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Studies Towards Understanding the Biosynthesis of Methyl Phosphoramidates found in Campylobacter jejuni
DownloadFall 2016
The human pathogen Campylobacter jejuni is the leading cause of food-borne gastroenteritis, causing more foodborne illnesses than Salmonella, Shigella, and Listeria combined. Of the approximate 5.3 million people infected every year in North America alone, approximately 1/1000 will develop the...
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Fall 2013
Solubility of glucose and lactose in subcritical water (SCW) was first determined at different pressures and temperatures. Sugar solubility increased with temperature but decreased with an increase in pressure. Then, carbohydrates and phenolics were extracted from lentil husk and cotyledon using...
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The APETALA-2-Like Transcription Factor OsAP2-39 Controls Key Interactions between Abscisic Acid and Gibberellin in Rice
Download2010
Rothstein, Steven J., Bi, Yong-Mei, Zhu, Tong, El-kereamy, Ashraf, Beatty, Perrin H., Yaish, Mahmoud W., Good, Allen G.
The interaction between phytohormones is an important mechanism which controls growth and developmental processes in plants. Deciphering these interactions is a crucial step in helping to develop crops with enhanced yield and resistance to environmental stresses. Controlling the expression level...