This week, we need to do work to address a component of our application that requires immediate attention. As a result, you may experience ERA to be intermittently available. We apologize for any inconvenience this may cause, and thank you for your patience as we attend to the care and feeding of ERA!
- 18 views
- 15 downloads
Structure and stability of carbohydrate-lipid interactions: Methylmannose polysaccharide-fatty acid complexes
-
We report a detailed study of the structure and stability of carbohydrate–lipid interactions. Complexes of a methylmannose polysaccharide (MMP) derivative and fatty acids (FAs) served as model systems. The dependence of solution affinities and gas-phase dissociation activation energies (Ea) on FA length indicates a dominant role of carbohydrate–lipid interactions in stabilizing (MMP+FA) complexes. Solution 1H NMR results reveal weak interactions between MMP methyl groups and FA acyl chain; MD simulations suggest the complexes are disordered. The contribution of FA methylene groups to the Ea is similar to that of heats of transfer of n-alkanes from the gas phase to polar solvents, thus suggesting that MMP binds lipids through dipole-induced dipole interactions. The MD results point to hydrophobic interactions and H-bonds with the FA carboxyl group. Comparison of collision cross sections of deprotonated (MMP+FA) ions with MD structures suggests that the gaseous complexes are disordered.
-
- Date created
- 2016
-
- Subjects / Keywords
-
- Type of Item
- Article (Published)
-
- License
- © 2016 Liu, L., Siuda, I., Richards, M. R., Renaud, J., Kitova, E. N., Mayer, P. M., Tieleman, D. P., Lowary, T. L., & Klassen, J. S. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.