Search
Skip to Search Results- 2Weselake, Randall J.
- 2Xu, Yang
- 1 Lemieux, M. Joanne
- 1Baral, Pravas Kumar
- 1Caldo, Kristian Mark P.
- 1Chen, Guanqun
- 9Graduate and Postdoctoral Studies (GPS), Faculty of
- 9Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 2Agricultural, Food and Nutritional Science, Department of
- 2Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Medicine and Dentistry, Faculty of
- 1Medicine and Dentistry, Faculty of/Journal Articles (Medicine and Dentistry)
-
Fall 2014
Quantitatively, nitrogen (N) has shown to be one of the most important nutrients for proper plant growth and development, and a key limiting factor in crop productivity. In the last 40 years, there has been a significant increase in the production of synthetic N-fertilizers, as well as an...
-
Biochemical Characterization of ATP-Binding Cassette Transporter G1 Mediated Cholesterol Efflux
DownloadSpring 2012
ABCG1 mediates cholesterol efflux onto lipidated apolipoprotein A-I and plays an important role in macrophage reverse cholesterol transport, thereby preventing atherosclerosis. However, how ABCG1 mediates cholesterol efflux is unclear. We found that Cys514 located within the third putative...
-
Fall 2014
Monoamine oxidase enzymes are largely involved in the catabolism of biogenic amines. Two forms of the enzyme are socumented to exist, monoamine oxidase type A and B. The B form (MAO-B) of the enzyme has been noted to possess a high affinity site for some imidazoline ligands. This site (the I2...
-
Spring 2017
Raman and Raman optical activity (ROA) spectroscopy are a set of especially effective spectroscopic practices for measuring the vibrational transitions of molecules in solution. They are particularly well suited for measurements of aqueous solutions because of the relatively little Raman...
-
Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
-
Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis
Download2011
Siloto, Rodrigo M. P., Snyder, Crystal L., Liu, Qin, Weselake, Randall J.
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...
-
Spring 2011
The mammalian Na+/H+ exchanger isoform 1 (NHE1) is a ubiquitous membrane protein that exchanges one intracellular H+ for an extracellular Na+, thereby regulating cell pH and volume. NHE1 catalytic activity is mediated by a transmembrane (TM) domain with 12 transmembrane segments. We performed...