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- 5Department of Chemistry
- 4Department of Electrical and Computer Engineering
- 3Department of Agricultural, Food, and Nutritional Science
- 1Department of Agricultural, Food and Nutritional Science
- 1Department of Biochemistry
- 1Department of Medical Microbiology and Infectious Diseases
- 2Kurgan, Lukasz (Electrical and Computer Engineering)
- 1Ball, Ronald O. (Department of Agricultural, Food and Nutritional Science)
- 1Ballermann, Barbara (Medicine)
- 1Boekhoven, Job (Chemistry, Technical University of Munich)
- 1D. Jed Harrison (Chemistry)
- 1Dr. Jianping Wu, Department of Agricultural,Food and Nutritional Sciences
The research presented in this work explores the application of microfluidics to the field of proteomics through the design of a multi-channel microfluidic platform and the investigation of individual components of the system. The design of this microfluidic device allows the integration of...
Structure and dynamics of biomolecules: probing muscle regulation, prion protein unfolding, and drug insertion into DNA by nuclear magnetic resonance spectroscopyDownload
Nuclear magnetic resonance (NMR) spectroscopy is a powerful approach to study the structure and dynamics of macromolecules in a close-to-native solution environment. In the present thesis I present my investigation of protein and nucleic acid structure and dynamics in a wide variety of biological...
Simultaneous measurement of protein and energy metabolism and application to determine lysine requirements in sowsDownload
Simultaneous measurements of energy and protein metabolism can provide valuable information about their interactions. Dietary lysine is limiting in typical feedstuffs fed to swine and, therefore, limits protein synthesis. Current recommendations for dietary amino acid and energy intakes may not...
An examination of linking and blocking procedures for use in deflection cantilever array-based protein detectionDownload
In this project common linking and blocking procedures were examined for use in multiplexed deflection cantilever array-based detection of proteins. A human interferon gamma (INF-γ) enzyme-linked immunosorbent assay (ELISA) kit was used to evaluate the effectiveness of the linking and blocking...
Proteins, which participate in virtually every process within cells, implement many of their functions through interactions with various ligands. Although a substantial effort in characterization and prediction of protein-ligand interactions was observed in the past two decades, these subjects...
Computational support systems for prediction and characterization of protein crystallization outcomesDownload
Analysis of protein structures may reveal their function, regulation and interactions. Almost 90% of the known protein structures were solved using X-ray crystallography; however, many more structures remain unsolved. Protein Structure Initiative (PSI) project was created to speed up structure...
The effects of broiler breeder dietary energy and protein during rearing and dietary energy level of lay diets on egg composition, offspring performance and carcass yield were evaluated. Pullets were fed a diet containing high ME (2,736 kcal/kg; HEREAR), or low ME (2,528 kcal/kg; LEREAR) combined...