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- 3Weselake, Randall J.
- 2Caldo, Kristian Mark P.
- 2Chen, Guanqun
- 2Greer, Michael S.
- 2Xu, Yang
- 8Graduate and Postdoctoral Studies (GPS), Faculty of
- 8Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 3Agricultural, Food and Nutritional Science, Department of
- 3Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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- 1WISEST Summer Research Program/WISEST Research Posters
- 3Deyholos, Michael (Biological Sciences)
- 1Ambrose, Divakar (Department of Agricultural, Food and Nutritional Science)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
- 1Davis, Corey (Biological Sciences)
- 1Ozga, Jocelyn (Department of Agricultural, Food and Nutritional Science)
- 1Rahman, Habibur (Department of Agricultural, Food & Nutritional Science)
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Broadening of genetic diversity in spring canola (Brassica napus L.) by use of yellow sarson and Canadian spring Brassica rapa L.
DownloadFall 2015
Canada is the top producer of Brassica oilseeds [B. napus L. (n = 19, AC genome)] in the world. Genetic diversity has declined in this crop in the recent years due to use of only superior and genetically narrow gene pool in breeding. Presence of adequate genetic diversity is important for further...
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Fall 2013
Lectins are proteins that bind specifically to carbohydrates and are active in plant defense and other processes. The sequencing of the flax (Linum usitatissimum) genome showed that it was unusually rich in genes predicted to encode one or more agglutinin domains (PFAM PF07468). This domain is...
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...
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STRESS RESPONSES OF THE FLAX GENOME: ACTIVATION OF TRANSPOSABLE ELEMENTS, DEFENSE GENES AND GENOME RESTRUCTURING AND DIVERSIFICATION.
DownloadSpring 2017
Transposable elements (TEs) are DNA sequences that can move in the genome (transpose) via a DNA or a RNA intermediate. TEs are abundant in plant genomes and can be activated by stress. Their activity can result in structural and gene expression alterations that increase diversity within and among...
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Fall 2011
Flax is an oilseed praised for high omega-3 oil, fiber and lignan content. Supercritical carbon dioxide (SCCO2) can be used as a green solvent alternative to process flax bioactives. SCCO2 extraction was used to remove lignan Secoisolariciresinol Diglucoside (SDG) from full fat and defatted whole...
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2021-08-01
Holly Pougnet, Eric Lepp, Ahmed Samir Ead, Dr. Jason Carey
Wind energy is a sustainable alternative to traditional fossil fuel sources. Improvements are constantly being made to maximize turbine efficiency, which is only increasing wind’s potential to be viable on a large-scale. Blades must be constructed using low density materials with high stiffness....
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Uncovering the Molecular Mechanisms behind Triacylglycerol Biosynthesis in Flaxseed (Linum usitatissimum L.)
DownloadFall 2015
Flax (Linum usitatissimum L.) seed oil contains approximately 45%-65% of α-linolenic acid (ALA) and 15-29 % of linoleic acid (LA), and is one of the richest natural sources of these polyunsaturated fatty acids (PUFAs). Due to its high amounts of PUFAs, especially ALA, flax oil has a number of...