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Skip to Search Results- 3Weselake, Randall J.
- 3Xu, Yang
- 2Caldo, Kristian Mark P.
- 2Chen, Guanqun
- 2Rahman, Habibur
- 2Siloto, Rodrigo M. P.
- 28Graduate and Postdoctoral Studies (GPS), Faculty of
- 28Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 6Agricultural, Food and Nutritional Science, Department of
- 6Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Medicine and Dentistry, Faculty of
- 1Medicine and Dentistry, Faculty of/Journal Articles (Medicine and Dentistry)
- 17Department of Agricultural, Food, and Nutritional Science
- 3Department of Biological Sciences
- 1Department of Agricultural, Food and Nutritional Science
- 1Department of Biochemistry
- 1Department of Botany
- 1Department of Chemical Engineering
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Allen Good (Biological Sciences)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
- 1Dosdall, Lloyd M. (Agricultural, Food and Nutritional Science)
- 1Dsupervisor: Dr. Habibur Rahman, AFNS Department, co-supervisor: Dr. Rong-Cai Yang (AFNS Department)
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Spring 2017
Raman and Raman optical activity (ROA) spectroscopy are a set of especially effective spectroscopic practices for measuring the vibrational transitions of molecules in solution. They are particularly well suited for measurements of aqueous solutions because of the relatively little Raman...
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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Evaluation of oilseed Brassica napus germplasm for days to flowering under a short-day condition and QTL mapping of the trait
DownloadSpring 2021
Canola (Brassica napus, AACC = 2n = 38) is an important oilseed crop in Canada. Among the different agronomic traits, the earliness of flowering and maturity are important for growing this crop on the Canadian prairies. The earliness of flowering can be improved by using the genes/alleles capable...
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Evaluation of Spring Canola (Brassica Napus) Lines Derived from Rutabaga (Brassica Napus Var. Napobrassica) X Canola Crosses for Agronomic and Seed Quality Traits and Heterosis
DownloadSpring 2018
Brassica napus L. (AACC, 2n = 38) canola is the most extensively cultivated Brassica oilseed crop in the world; it is one of the most important field crops in Canada. The narrow genetic diversity in this crop is considered one of the major hindrances for continued improvement of this crop for...
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Functional and topological analysis of yeast acyl-CoA:diacylglycerol acyltransferase 2, an endoplasmic reticulum enzyme essential for triacylglycerol biosynthesis
Download2011
Siloto, Rodrigo M. P., Snyder, Crystal L., Liu, Qin, Weselake, Randall J.
Acyl-CoA:diacylglycerol acyltransferase (EC 2.3.1.20) is a membrane protein present mainly in the endoplasmic reticulum. It catalyzes the final and committed step in the biosynthesis of triacylglycerol, which is the principal repository of fatty acids for energy utilization and membrane...
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Spring 2011
The mammalian Na+/H+ exchanger isoform 1 (NHE1) is a ubiquitous membrane protein that exchanges one intracellular H+ for an extracellular Na+, thereby regulating cell pH and volume. NHE1 catalytic activity is mediated by a transmembrane (TM) domain with 12 transmembrane segments. We performed...
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Genetic and molecular analysis of clubroot resistance in canola introgressed from rutabaga cvs. Polycross and Brookfield
DownloadFall 2022
Clubroot disease caused by Plasmodiophora brassicae is a serious threat to canola (Brassica napus) production. The evolution of new pathotypes has rendered available resistances ineffective and has necessitated the introgression of new resistance into canola and furthering our understanding of...
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Genetic study on Brassica rapa and Brassica napus for seed color and identification of molecular markers
DownloadFall 2012
Yellow seed in Brassica oilseed crops is favoured for its association with lower fibre and higher oil contents. Yellow-seeded Brassica napus lines have been developed through introgression of this trait from its allied species; however, the trait in B. napus is often influenced by environmental...
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...