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Skip to Search Results- 5Chen, Guanqun
- 5Weselake, Randall J.
- 4Caldo, Kristian Mark P.
- 4Xu, Yang
- 3Greer, Michael S.
- 3Mietkiewska, Elzbieta
- 34Graduate and Postdoctoral Studies (GPS), Faculty of
- 34Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 13Agricultural, Food and Nutritional Science, Department of
- 13Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Biological Sciences, Department of
- 1Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 22Department of Agricultural, Food, and Nutritional Science
- 3Department of Biochemistry
- 3Department of Biological Sciences
- 1Department of Agricultural, Food and Nutritional Science
- 1Department of Botany
- 1Department of Chemical Engineering
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Jacobs, Rene (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 2Vance, Dennis E. (Biochemistry)
- 1Allen Good (Biological Sciences)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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Impaired de novo choline synthesis explains why phosphatidylethanolamine N-methyltransferase-deficient mice are protected from diet-induced obesity
Download2010
Kuo, Ming-Shang, Dyck, Jason R. B., Sletten, Torunn, Proctor, Spencer D., Jacobs, René L., Lingrell, Susanne, Cao, Guoqing, Peake, David A., Kennedy, Brian P., Zhao, Yang, Vance, Dennis E., Koonen, Debby P. Y., Su, Brian
Phosphatidylcholine (PC) is synthesized from choline via the CDP-choline pathway. Liver cells can also synthesize PC via the sequential methylation of phosphatidylethanolamine, catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). The current study investigates whether or not hepatic...
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Spring 2013
Egg yolk contains approximately 10% (w/w) phospholipids (PL), of which about 70% is phosphatidylcholine (PC). Conventional methods of PL extraction from egg yolk involve using hazardous organic solvents. Supercritical carbon dioxide (SC-CO2), as a "green" solvent, had been applied for the...
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...
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Improving Verticillium longisporum inoculation protocols and quantifying canola yield losses
DownloadSpring 2024
Verticillium stripe, caused by Verticillium longisporum, is an emerging soilborne disease of the Canadian canola (Brassica napus) crop. This study aimed to refine techniques for inoculating the pathogen on canola and to quantify its impact on hybrid canola yield under greenhouse and field...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Insights into the role of CTP:phosphocholine cytidylyltransferase-alpha in hepatic lipid metabolism and cellular integrity
DownloadFall 2011
Lower levels of hepatic phosphatidylcholine (PC) are suggested to play a role in the pathogenesis of non-alcoholic steatohepatitis (NASH). Mice lacking hepatic CTP:phosphocholine cytidylyltransferase-α (LCTα-/-), the regulatory enzyme in the CDP-choline pathway for PC biosynthesis, were fed a...
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Insights into the Transcriptional Regulation and Physiological Importance of Phosphatidylethanolamine N-Methyltransferase
DownloadSpring 2010
Phosphatidylcholine (PC) is made in all nucleated mammalian cells via the CDP-choline pathway. Another major pathway for PC biosynthesis in liver is catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). We have identified 3T3-L1 adipocytes as a cell culture model that expresses PEMT...
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Introgression of Clubroot Resistance from Brassica oleracea into B. napus Canola through Interspecific Cross
DownloadSpring 2022
Clubroot disease caused by Plasmodiophora brassicae Woronin is a serious threat to canola production in Canada. Most of the available clubroot-resistant canola cultivars carry major resistance genes of the Brassica A genome. However, the ineffectiveness of this type of resistance has been...