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Skip to Search Results- 5Chen, Guanqun
- 5Weselake, Randall J.
- 4Caldo, Kristian Mark P.
- 4Xu, Yang
- 3Greer, Michael S.
- 3Mietkiewska, Elzbieta
- 26Graduate and Postdoctoral Studies (GPS), Faculty of
- 26Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 10Agricultural, Food and Nutritional Science, Department of
- 10Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 2Biological Sciences, Department of
- 2Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 18Department of Agricultural, Food, and Nutritional Science
- 2Department of Biological Sciences
- 1Department of Agricultural, Food and Nutritional Science
- 1Department of Anatomy and Cell Biology
- 1Department of Biochemistry
- 1Department of Cell Biology
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
- 1Dosdall, Lloyd M. (Agricultural, Food and Nutritional Science)
- 1Douglas, Donna (Department of Surgery)
- 1Dsupervisor: Dr. Habibur Rahman, AFNS Department, co-supervisor: Dr. Rong-Cai Yang (AFNS Department)
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Evaluation of Spring Canola (Brassica Napus) Lines Derived from Rutabaga (Brassica Napus Var. Napobrassica) X Canola Crosses for Agronomic and Seed Quality Traits and Heterosis
DownloadSpring 2018
Brassica napus L. (AACC, 2n = 38) canola is the most extensively cultivated Brassica oilseed crop in the world; it is one of the most important field crops in Canada. The narrow genetic diversity in this crop is considered one of the major hindrances for continued improvement of this crop for...
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Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
Download2007
Pozniakovsky, A., Hyman, A.A., Stirling, P.C., Takhar, K.S., Srayko, M., Lerou, M.R.
The Chaperonin Containing Tcp1 (CCT) maintains cellular protein folding homeostasis in the eukaryotic cytosol by assisting the biogenesis of many proteins, including actins, tubulins, and regulators of the cell cycle. Here, we demonstrate that the essential and conserved eukaryotic phosducin-like...
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Genetic and molecular analysis of clubroot resistance in canola introgressed from rutabaga cvs. Polycross and Brookfield
DownloadFall 2022
Clubroot disease caused by Plasmodiophora brassicae is a serious threat to canola (Brassica napus) production. The evolution of new pathotypes has rendered available resistances ineffective and has necessitated the introgression of new resistance into canola and furthering our understanding of...
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Genetic study on Brassica rapa and Brassica napus for seed color and identification of molecular markers
DownloadFall 2012
Yellow seed in Brassica oilseed crops is favoured for its association with lower fibre and higher oil contents. Yellow-seeded Brassica napus lines have been developed through introgression of this trait from its allied species; however, the trait in B. napus is often influenced by environmental...
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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Spring 2014
Rho GTPases act as molecular switches, occupying an active GTP or an inactive GDP-bound state. Rho1p, one member of the Rho family GTPases, has a role in the late stages of vacuole fusion through an unknown mechanism. We identified a ~50kDa Rho1p-interacting protein as Tef1p, a dual function...
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...
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Improving Verticillium longisporum inoculation protocols and quantifying canola yield losses
DownloadSpring 2024
Verticillium stripe, caused by Verticillium longisporum, is an emerging soilborne disease of the Canadian canola (Brassica napus) crop. This study aimed to refine techniques for inoculating the pathogen on canola and to quantify its impact on hybrid canola yield under greenhouse and field...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...