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Gene co-expression network analysis identifies porcine genes associated with variation in Salmonella shedding
Download2014
Plastow, G. S., Bearson, S. M. D., Arantes, A. S., Bao, H., Kommadath, A., Stothard, P., Guan, L. L., Tuggle, C. K.
Background Salmonella enterica serovar Typhimurium is a gram-negative bacterium that can colonise the gut of humans and several species of food producing farm animals to cause enteric or septicaemic salmonellosis. While many studies have looked into the host genetic response to Salmonella...
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Genetic parameters for production and feeding behaviour traits in crossbred steers fed a finishing diet at different ages
Download2013
Wang, Z., Nkrumah, D. J., Basarab, J. A., Durunna, O. N., Okine, E. K., Moore, S. S., Mujibi, F. D. N.
Because cattle can be raised postweaning under several feeding regimes, this study examined the consistency of phenotypic and genetic parameters of some production and feeding behaviour traits between two feeding periods that beef cattle received a finisher diet. Crossbred steers (n=851) were...
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2001
Ciobanu, D. C., Nagy, A., Day, A. E., Plastow, G. S., Rothschild, M. F., Wales, R.
Analysis of the genetic variation of an endangered population is an important component for the success of conservation. Animals from two local Romanian pig breeds, the Mangalitsa and Bazna, were analysed for variation at a number of genetic loci using PCR-based DNA tests. Polymorphism was...
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Genome-wide association studies (GWAS) identify a QTL close to PRKAG3 affecting meat pH and colour in crossbred commercial pigs
Download2015
Miar, Y., Plastow, G. S., Charagu, P., Wang, Z., Kemp, R. A., Bruce, H., Zhang, Z., Yang, T.
Background Improving meat quality is a high priority for the pork industry to satisfy consumers’ preferences. GWAS have become a state-of-the-art approach to genetically improve economically important traits. However, GWAS focused on pork quality are still relatively rare. Results Six...
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Greenhouse gas emissions from calf- and yearling-fed beef production systems, with and without the use of growth promotants
Download2012
Baron, V. S, Aalhus, J. L., Basarab, J. A., López-Campos, Ó., Haugen-Kozyra, K., Okine, E. K.
A spring calving herd consisting of about 350 beef cows, 14–16 breeding bulls, 60 replacement heifers and 112 steers were used to compare the whole-farm GHG emissions among calf-fed vs. yearling-fed production systems with and without growth implants. Carbon footprint ranged from 11.63 to 13.22...
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Identification of a putative quantitative trait nucleotide in guanylate binding protein 5 for host response to PRRS virus infection
Download2015
Choi, I., Guan, L., Plastow, G. S., Liu, P., Koltes, J. E., Loyd, H., Boddicker, N. J., Eisley, C. J., Schroyen, M., Bao, H., Dekkers, J. C. M., Kommadath, A., Abrams, S. M., Reecy, J. M., Rowland, R. R. R., Serão, N. V. L., Lunney, J. K., Carpenter, S., Stothard, P., Fritz-Waters, E., Tuggle, C. K.
Background Previously, we identified a major quantitative trait locus (QTL) for host response to Porcine Respiratory and Reproductive Syndrome virus (PRRSV) infection in high linkage disequilibrium (LD) with SNP rs80800372 on Sus scrofa chromosome 4 (SSC4). Results Within this QTL,...
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Increasing gene discovery and coverage using RNA-seq of globin RNA reduced porcine blood samples
Download2014
Stothard, P., Fritz-Waters, E., Choi, I., Abrams, S. M., Kommadath, A., Hosseini, A., Plastow, G. S., Guan, L. L., Tuggle, C. K., Bao, H., Meng, Y., Lunney, J. K., Reecy, J. M., Sun, X.
Background Transcriptome analysis of porcine whole blood has several applications, which include deciphering genetic mechanisms for host responses to viral infection and vaccination. The abundance of alpha- and beta-globin transcripts in blood, however, impedes the ability to cost-effectively...
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2003
Chanmugam, P. S., Okine, E. K., Wang, Z., Mir, P. S., Goonewardene, L. A., Mir, Z.
Four Alpine does were used in a 4 × 4 Latin square design to determine the effects of feeding canola oil at four levels: 0, 2, 4 and 6% on milk fatty acid profile. The sum of C12:0 + C14:0 + C16:0(hypercholesterolemic fatty acids) decreased linearly and C18:0 + C18:1: C16:0 (indicator of...
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1988
Plastow, G. S., Mileham, A. J., Siggens, K. W.
A 3.8 kb male specific fragment was observed following agarose gel etectrophoresis of porcine DNA digested with Sph I. DNA was recovered from this region of a gel using DE-81 paper and ligated to Sph I digested pUC18. Plasmids carrying male specific sequences were identified by their differential...
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MicroRNA Buffering and Altered Variance of Gene Expression in Response to Salmonella Infection
Download2014
Plastow, G. S., Bao, H., Kommadath, A., Guan, L. L., Tuggle, C. K., Stothard, P.
One potential role of miRNAs is to buffer variation in gene expression, although conflicting results have been reported. To investigate the buffering role of miRNAs in response to Salmonella infection in pigs, we sequenced miRNA and mRNA in whole blood from 15 pig samples before and after...