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Molecular Prognostication of Oral Cavity Squamous Cell Carcinoma by Tissue Microarray Analysis
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- Author / Creator
- Alenazi, Abdulrahman, F
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AimsOral cavity squamous cell carcinoma (OCSCC) is a molecularly heterogeneous disease, which is thought to account for differences in treatment response between patients who have otherwise similar characteristics. Cancer biomarkers provide a means of molecular classification, which can be of diagnostic and prognostic utility. This study aims to investigate the prognostic value of a panel of established biomarkers in OCSCC.MethodsUsing a prospectively collected dataset, patients with OCSCC diagnosed and treated during 1998-2010 were identified for study inclusion. Formalin-fixed paraffin embedded tumors from these patients were obtained for the construction tissue microarrays (TMAs), which were stained by immunohistochemistry with p16, p53, Bcl-xL, EGFR, Ki67, pancytokeratin, and DAPI. Each biomarker was measured using quantitative immunofluorescence, within tumor nuclear and cytoplasmic compartments, relative to normal control tissue controls. Biomarker expression levels were correlated with patient survival outcomes using univariate and multivariate analyses.ResultsA total of 254 OCSCC patients were identified of which 187 had adequate tissue for TMA construction. All the included patients had been treated with surgical modalities. Negative Ki67 expression was associated with improved disease specific survival (p = 0.02). High EGFR or high p53 expression were associated with significantly lower survival outcomes (p = 0.02 and 0.034 respectively). P16 and Bcl-xL levels were not predictive of survival.ConclusionsOCSCC patients with negative Ki67 have significantly higher survival outcomes, while high EGFR or high p53 have significantly lower survival outcomes. These biomarkers may be predictive of more aggressive pathology and/or treatment resistance in OCSCC.
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- Graduation date
- Spring 2018
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.