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Exploring Novel Methods in Sonoportation

  • Author / Creator
    Wong, Peter Kwok Pan
  • Sonoporation, a physical, non-viral, non-chemical transfection method, promises great potentials. However, many drawbacks hinders its generalization. Low transfection rate and cell viability after treatment are among the hindering factors of sonoporation. The purpose of the research performed in this thesis was to develop, explore and analyze new methodologies to overcome the known drawbacks of sonoporation, which are to increase cell viability and transfection rate. These novel methods include the use of a self-developed ultrasound box, self-developed microbubble carriers and the synergistic use of chemical transfection reagents. Sonoporation were performed on MCF-7 and KG-1 cells as they represent easy and difficult to transfect cell lines respectively. Permeability markers, flow cytometry, MTT assay and MTS assay were used to quantify transfection rate and cell viability after sonoporation. New procedures were performed, analyzed and evaluated for their feasibility for drug and or gene delivery. The thesis has shown improvements in transfection rate and preserving viability. However, sonoporation still remains an inefficient method to deliver material into hard-to-transfect cells.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Biomedical Engineering
  • Supervisor / co-supervisor and their department(s)
    • Chen, Jie (Electrical Engineering/Biomedical Engineering)
  • Examining committee members and their departments
    • Li, Lawrence (Biomedical Engineering)
    • Xing, James (Laboratory Medicine and Pathology)