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- 2Gene therapy
- 2Non-viral gene delivery
- 1Cationic polymers
- 1Cell-based therapy
- 1Abbasi Dezfouli, Meysam
- 1Abdulrahman, Nabeel
- 1Filmore, Natasha
- 1Fliegel, Larry
- 1Gadeau, Alain-Pierre
- 1Gainer, Anita Lillian.
Sonoporation, a physical, non-viral, non-chemical transfection method, promises great potentials. However, many drawbacks hinders its generalization. Low transfection rate and cell viability after treatment are among the hindering factors of sonoporation. The purpose of the research performed...
Lipid Modified Polymers for Transfection of Human CRL Fibroblasts, and for siRNA Mediated MDR Reversal in Melanoma Cancer TherapyDownload
Gene delivery for therapeutic purposes is quickly emerging as the best potential treatment option for inherited genetic diseases and cancer. Viral gene carriers have been the choice for this purpose due to their high efficiency, but harmful immunogenic and oncogenic host reactions have limited...
Mechanistic studies on the uptake and intracellular trafficking of DNA complexes in primary cells using lipid-modified cationic polymers as non-viral gene carrierDownload
This thesis work is aimed at addressing some of the fundamental questions surrounding the intracellular fate of plasmid DNA in clinically-relevant primary cells, when delivered with lipid-modi!ed cationic polymers as gene carriers. We developed an optimized procedure for the transfection of...
Enhanced expression and activity of the Na+/H+ exchanger isoform 1 (NHE1) has been implicated in cardiomyocyte hypertrophy in various experimental models. The upregulation of NHE1 was correlated with an increase in osteopontin (OPN) expression in models of cardiac hypertrophy (CH), and the...