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RESEARCHOpen AccessDevelopment of a 3D printed device tosupport long term intestinal culture as analternative to hyperoxic chamber methods

  • Author(s) / Creator(s)
  • Background:Most interactions between pathogenic microorganisms and their target host occur on mucosal surfaces of internal organs such as the intestine. In vitro organ culture (IVOC) provides an unique tool for studyinghost-pathogen interactions in a controlled environment. However, this technique requires a complex laboratory setup and specialized apparatus. In addition, issues arise when anaerobic pathogens are exposed to the hyperoxic environment required for intestinal culture. The objective of this study was to develop an accessible 3D–printed device that would allow manipulation of the gas mixture used to supply the tissue culture media separately from the gas mixture exposed to the mucosal side of explants. Results:Porcine colon explants from 2 pigs were prepared (n= 20) and cultured for 0h, 8h, 18h and 24h using the device. After the culture period, explants were fixed in formalin and H&E stained sections were evaluated for histological defects of the mucosa. At 8h, 66% of samples displayed no histological abnormalities, whereas samples collected at 18hand 24h displayed progressively increasing rates of superficial epithelial erosion and epithelial metaplasia.Conclusions:The 3D–design reported here allows investigators to setup intestinal culture explants while manipulating the gas media explants are exposed to, to support tissue viability for a minimal of 8h. The amount of media necessary and tissue contamination are potential issues associated with this apparatus

  • Date created
    2017-01-01
  • Subjects / Keywords
  • Type of Item
    Article (Published)
  • DOI
    https://doi.org/10.7939/r3-0ymn-6249
  • License
    Attribution-NonCommercial 4.0 International
  • Language
  • Citation for previous publication
    • Matheus O. Costa, Roman Nosach, & John C.S. Harding. (2017). Development of a 3D printed device to support long term intestinal culture as an alternative to hyperoxic chamber methods. 3D Printing in Medicine, 3(1), 1–5. https://doi-org.login.ezproxy.library.ualberta.ca/10.1186/s41205-017-0018-z