Usage
  • 33 views
  • 35 downloads

Dietary Gangliosides Modify Helicobacter pylori Adhesion to Human Gastric Epithelial Cells

  • Author / Creator
    Rivas Serna, Irma M
  • This research determined if alteration of plasma membrane by exogenous gangliosides inhibits Helicobacter pylori adhesion to human gastric epithelial cells. The objective was to evaluate the composition of gangliosides from bovine whole raw milk, bovine colostrum and powder buttermilk using Liquid Chromatography/Mass Spectrometry (LC/MS). This research determined if alteration of the GM3 and GD3 content of the apical and basolateral membrane of a human gastric cell line was modified by exogenous gangliosides. The effect of ganglioside treatment on the composition and content of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) and on H. pylori adhesion was assessed. A rapid and sensitive LC/MS method was established and used to determine content and composition of gangliosides. Ganglioside composition was analyzed in three different milk products (raw milk, colostrum, buttermilk). GD3 was found to be the most abundant ganglioside species. Human gastric cells fed with exogenous GM3 and GD3 modified ganglioside content, but most abundant ganglioside was GM3. GM3 was found in the apical side and GD3 in the basolateral side of the cell. After ganglioside treatments, composition of ganglioside content of membrane domains was modified. Composition and content of phospholipids was not altered after ganglioside treatment. Gastric cells treated with gangliosides decreased H. pylori adhesion, but concentration of these gangliosides was not crucial for reduction. GM3 and GD3 decrease H. pylori adhesion. The combination of GM3:GD3 also decreased H. pylori adhesion suggesting that human and bovine milk might decrease H. pylori adhesion. This thesis demonstrates that 1) gangliosides are taken up in gastric cells, 2) gangliosides fed from the apical side also affect content of gangliosides in the basolateral side, and 3) GD3 and GM3 reduce H. pylori adhesion to human gastric cells. This research suggests that gangliosides may be an anti-adhesion strategy to decrease or prevent H. pylori infection.

  • Subjects / Keywords
  • Graduation date
    2015-06
  • Type of Item
    Thesis
  • Degree
    Doctor of Philosophy
  • DOI
    https://doi.org/10.7939/R39Z90J8R
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Doctoral
  • Department
    • Department of Agricultural, Food, and Nutritional Science
  • Specialization
    • Nutrition and Metabolism
  • Supervisor / co-supervisor and their department(s)
    • Clandinin, Tom (AFNS)
  • Examining committee members and their departments
    • Keelan, Monika (Laboratory of Medicine and Pathology)
    • Schnabl, Kareena (U of A Hospital)
    • Clandinin, Tom (AFNS)
    • Mazurak, Vera (AFNS)
    • Jones, Peter (Food Science U of M)
    • Lynn McMullen (AFNS)