Elevated Fatty Acid Content in Muscle is Prevented by EPA and DHA in an Animal Model of Colorectal Cancer Receiving CPT-11 / 5-FU

• Author / Creator

  Almasud, Alaa A

• This study aimed to assess the effect of irinotecan / 5-fluorouracil treatment on amount and types of fatty acids in skeletal muscles of tumor-bearing rats fed: a) control diet, b) fish oil diet. Rats bearing the Ward colorectal carcinoma were fed a semi-purified diet with or without fish oil prior to receiving chemotherapy. Gastrocnemius muscles were isolated 7 days later. Lipids were extracted and triglycerides (TG) and phospholipid (PL) separated by thin layer chromatography, and fatty acids (FAs) quantified by gas liquid chromatography. Rats receiving chemotherapy exhibited the most TG-FA in muscle tissue. Rats fed the fish oil diet exhibited lower total muscle TG compared with other groups. N-3 FA content in muscle TG and PL were higher in the fish oil group compared to other groups. This study suggests that fish oil modifies the fat accumulation in muscle that occurs during chemotherapy.
   

• Subjects / Keywords

  • Essential Fatty Acids
  • Skeletal muscles
  • Colorectal Cancer
  • Sarcopenia
  • Irinotecan / 5-fluorouracil
  • Cachexia
  • EPA and DHA

• Graduation date

  Fall 2012

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3TH0G

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.