A tale of two hypotheses: Effect of the herpes simplex-1 virus virion host shutoff (vhs) protein on bicistronic reporters

  • Author / Creator
    Rose, Alexandra R
  • The herpes simplex virus virion host shutoff (vhs) protein acts as an mRNA specific endoribonuclease. Our lab has previously determined that vhs stimulates expression of the 3’ CAT cistron of a bicistronic reporter plasmid while under the influence of the BiP IRES; however, the mechanism by which this occurs is unclear. Thus, different reporter constructs were assayed for CAT activity while under the influence of vhs. Vhs-induced CAT activation occurred not only in bicistronic reporter constructs containing the BiP IRES, but also in those containing truncated versions of the BiP IRES or an exonuclease blocking RNA element. The unrelated viral host shutoff nucleases EBV BGLF5, KHSV SOX and SARS coronavirus Nsp1 were also capable of stimulating CAT activity. Finally, a loss of mRNA was detected using northern blot after addition of vhs. Thus, it appears as if vhs activates 3’ cistron expression by degrading the 5’ cistron of the bicistronic mRNA instead of activating cap-independent translation.

  • Subjects / Keywords
  • Graduation date
    Fall 2014
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Specialization
    • Virology
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Schultz, Michael (Biochemistry)
    • Smiley, Jim (Med Micro and Immunology)
    • Shmulevitz, Maya (Med Micro and Immunology)
    • Baldwin, Troy (Med Micro and Immunology)
    • Hazes, Bart (Med Micro and Immunology)