Effects of Hyperthermia and Subsequent Minocycline Treatment in Acute Ischemic Stroke

  • Author / Creator
    Rahman, Shakib Hafizur
  • Stroke, a reduction in blood flow to the brain, activates various proteins that contribute to neuronal death. The matrix metalloproteinases (MMPs), specifically MMP2 and MMP9, are important in maintaining the blood brain barrier (BBB). After a stroke the BBB permeability increases which is caused by MMP upregulation. Hyperthermia is known to exacerbate the damage caused by a stroke, but the exact mechanism is unknown. We hypothesize that hyperthermia will increase MMP expression and that minocycline, an MMP inhibitor, will decrease MMP activity and BBB permeability. Our data demonstrates that hyperthermia increases expression of both MMP2 and MMP9. Furthermore, hyperthermia after ischemia increases degradation of laminin. Minocycline can reduce both expression and activity of MMP2 and MMP9 after ischemia. Minocycline is effective in hyperthermic conditions and reduces degradation of laminin. This data shows that minocycline is a potential therapeutic treatment for reducing MMP activity and subsequent laminin degradation after a stroke.

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  • Degree
    Master of Science
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