Effects of Hyperthermia and Subsequent Minocycline Treatment in Acute Ischemic Stroke

• Author / Creator

  Rahman, Shakib Hafizur

• Stroke, a reduction in blood flow to the brain, activates various proteins that contribute to neuronal death. The matrix metalloproteinases (MMPs), specifically MMP2 and MMP9, are important in maintaining the blood brain barrier (BBB). After a stroke the BBB permeability increases which is caused by MMP upregulation. Hyperthermia is known to exacerbate the damage caused by a stroke, but the exact mechanism is unknown. We hypothesize that hyperthermia will increase MMP expression and that minocycline, an MMP inhibitor, will decrease MMP activity and BBB permeability. Our data demonstrates that hyperthermia increases expression of both MMP2 and MMP9. Furthermore, hyperthermia after ischemia increases degradation of laminin. Minocycline can reduce both expression and activity of MMP2 and MMP9 after ischemia. Minocycline is effective in hyperthermic conditions and reduces degradation of laminin. This data shows that minocycline is a potential therapeutic treatment for reducing MMP activity and subsequent laminin degradation after a stroke.

• Subjects / Keywords

  • Ischemia
  • Minocycline
  • Matrix metalloprotease
  • Stroke
  • Laminin
  • Hyperthermia

• Graduation date

  Spring 2012

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3406C

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.