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Characterisation of novel potential phosphorylation sites in the tumour suppressor Merlin in Drosophila

  • Author / Creator
    Yip, Sophia
  • Neurofibromatosis Type II is an inherited cancer that manifests as various tumours in the nervous system. Mutations and deletions in the tumour suppressor Merlin (moesin, ezrin, radixin-like protein) are linked to the development of the disease. The mechanism by which Merlin suppresses cell growth is not yet clearly understood, however studies have shown that phosphorylation plays an important role in regulating the sub-cellular localisation, conformation, and activity of Merlin. Using in vivo genetic methods in Drosophila melanogaster, I undertook a characterisation of two novel potential phosphorylation sites that appear to be regulating Merlin function. In this thesis, I show that two phosphorylatable residues, serine 371 and threonine 18, in Drosophila Merlin affect the sub-cellular localisation and function of the Merlin protein.

  • Subjects / Keywords
  • Graduation date
    2014-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3CJ87X19
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Medical Sciences-Medical Genetics
  • Supervisor / co-supervisor and their department(s)
    • Hughes, Sarah (Medical Genetics)
  • Examining committee members and their departments
    • Eisenstat, David (Pediatrics)
    • Campbell, Shelagh (Biological Sciences)
    • Wevrick, Rachel (Medical Genetics)
    • Yokota, Toshifumi (Medical Genetics)