T cell-mediated inflammation is stereotyped: mouse delayed-type hypersensitivity reaction and mouse T cell-mediated rejection of renal allografts share common molecular mechanismsT cell-mediated inflammation is stereotyped

  • Author / Creator
    Venner, Jeffery
  • Genome-wide gene expression analysis of diseases has revealed large-scale changes in the expression of thousands of genes (transcripts) representing biological processes. The processes that occur during T cell-mediated rejection (TCMR) of renal allografts in mice and humans have been previously delineated, and they appear to be independent of cytotoxic mechanisms; thus, TCMR is analogous to delayed-type hypersensitivity (DTH). Since TCMR and DTH involve similar mechanisms, we hypothesized the molecular changes in TCMR are stereotyped; thus, they are qualitatively the same as those in DTH. Using microarrays to compare the transcript expression changes of both diseases in mice, we found they share the same processes: T cell and macrophage infiltration, IFNG-effects, alternative macrophage activation, and a coordinated injury-repair response of the tissue parenchyma. Additional analysis revealed IFNG is vital for stabilizing the injury-repair response in TCMR and DTH. We conclude the molecular changes in TCMR and DTH involve stereotyped, coordinated processes.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Medicine
  • Supervisor / co-supervisor and their department(s)
    • Halloran, Philip (Medicine)
    • Mengel, Michael (Laboratory Medicine and Pathology)
  • Examining committee members and their departments
    • Anderson, Colin (Surgery)