Intestinal Uptake of Barley Protein Nanoparticles as Delivery Vehicles for Bioactive Compounds

• Author / Creator

  Zhou, Ying

• The use of nanoparticles as nutrient delivery vehicles enables the enhancement of the oral bioavailability and health promoting benefits of bioactive compounds. Barley protein nanoparticles were developed in previous study for hydrophobic compound delivery. The objective of the present study was to evaluate the cytotoxicity of the nanoparticles and to characterize their intestinal uptake properties using in vitro and ex vivo models. The nanoparticles showed low cytotoxicity in Caco-2 cells. Their cellular uptake was dependent on time, concentration and temperature, suggesting transcytosis pathway. Significantly greater β-carotene uptake (15%) was observed in Caco-2 cells when delivered by nanoparticles compared to control (2.6%). The nanoparticles also showed adhesion and permeation abilities in rat jejunum tissues. Findings from this study demonstrated the uptake improving effect of barley protein nanoparticles and suggested their potential as nutrient delivery vehicles for the development of novel functional foods.

• Subjects / Keywords

  • Barley protein
  • Uptake
  • Nanoparticles
  • Bioavailability
  • Caco-2 cells

• Graduation date

  Fall 2013

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R31C1TS31

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.